Targeted genome editing using engineered CRISPR-Cas endonucleases

该项目旨在研究靶向和细胞特异性核酸内切酶的产生。CRISPR-Cas内切酶以其基于短指导RNA的编程简单性和高切割效率而彻底改变了基因组工程领域。该项目将结合基因组工程和抗体疗法中的两项技术来产生新的抗体靶向内切酶，以高效和特异性地修饰细胞基因组。该项目将提供对酒窖功能的新的和有趣的见解，在基础研究和生物技术中具有广泛的应用。

This project aims to study the generation of targeted and cell-specific endonucleases. CRISPR-Cas endonucleases have revolutionised the field of genome engineering due to programming simplicity based on a short guide RNA and high cleavage efficiency. This project will combine the use of two technologies in genome engineering and antibody therapeutics to generate new antibody-targeted endonucleases that modify cellular genomes with high efficacy and specificity. This project will provide new and intriguing insights into cellar function, with broad applications in basic research and biotechnology.