CHEMICAL SYNTHESIS AND STRUCTURE OF BACTERIAL POLYSACCHARIDES AND CONJUGATES

荚膜多糖是致病菌的重要毒力因子 细菌，是对抗细菌疾病的免疫原。的目标是 这个项目是为了了解这种多糖的化学和 利用这种化学物质来(A)研制针对 细菌病和(B)了解这些细菌的结构 糖偶联物以及它们如何与免疫系统相互作用。基于 这种方法正在开发一种化学酶法来制备 免疫原性差的大肠杆菌K1多唾液酸胞外多糖类似物 ACED。唾液酸的类似物已经被合成并被喂给 缺乏合成能力的多糖产生菌 唾液酸。所产生的免疫化学特征 正在测定多糖类。

Capsular polysaccharides are important virulence factors for pathogenic bacteria and are immunogens against bacterial disease. The objective of this project is to understand the chemistry of this polysaccharide and to use this chemistry to (a) develop glyconconjugate vaccines against bacterial disease and (b) understand the structure of these glycoconjugates and how they interact with the immune system. Based on this approach a chemoenzymatic method is being developed to prepare polysaccharide analogs of the poorly immunogenic E. coli K1 polysialic acied. Analogs of sialic acid have been synthesized and feed to polysaccharide producing bacteria lacking the ability to synthesize sialic acid. The immunochemical characteristics of the resulting polysaccharides are being determined.