CHEMICAL SYNTHESIS AND STRUCTURE OF BACTERIAL POLYSACCHA

荚膜多糖是病原菌的重要毒力因子 细菌，是抵抗细菌性疾病的免疫原。 的目标 这个项目是为了了解这种多糖的化学性质， 使用这种化学方法来（a）开发针对以下疾病的糖缀合物疫苗： （B）了解这些细菌的结构 糖缀合物以及它们如何与免疫系统相互作用。 基于 这种方法是一种化学酶的方法正在开发， 免疫原性差的E.大肠杆菌K1多聚唾液酸 acied. 已经合成了唾液酸的类似物，并将其进料至 缺乏合成唾液酸能力的产多糖细菌 酸 所得多糖的免疫化学特性 正在被确定。

Capsular polysaccharides are important virulence factors for pathogenic bacteria and are immunogens against bacterial disease. The objective of this project is to understand the chemistry of this polysaccharide and to use this chemistry to (a) develop glyconconjugate vaccines against bacterial disease and (b) understand the structure of these glycoconjugates and how they interact with the immune system. Based on this approach a chemoenzymatic method is being developed to prepare polysaccharide analogs of the poorly immunogenic E. coli K1 polysialic acied. Analogs of sialic acid have been synthesized and feed to polysaccharide producing bacteria lacking the ability to synthesize sialic acid. The immunochemical characteristics of the resulting polysaccharides are being determined.