PREVALENCE OF HEPATITIS G VIRUS

庚型肝炎病毒(HGV)是一种新近发现的RNA病毒 通过非肠道暴露于血液和血液制品而传播，以及 可能与人类的临床肝炎有关。的目标是 本研究旨在评估丙型肝炎病毒感染者中庚型肝炎病毒的流行情况。 个体和丙型肝炎病毒感染的免疫球蛋白静脉注射(IGIV) 产品。不同的套式引物，专用于5‘ HGV的非编码区被反向设计和测试 转录和聚合酶链式反应(RT-PCR)检测。这一组 被发现在检测HGV方面最有效的引物用于 检测慢性感染丙型肝炎病毒阳性的血清样本(丙型肝炎病毒2.0酶联免疫吸附试验， RIBA 2.0和HCVRNA阳性)患者和6例HCVRNA阳性IGIV批次。 对扩增产物进行测序和鉴定，证实了HGV的特异性。 将它们与已公布的HGV序列进行比较。检测到HGV RNA 在50例(28%)感染丙型肝炎病毒的患者血清样本中，有14例(28%)，在 6个(33%)IGIV批次。这些结果表明HGV rna是相当普遍的。 在丙型肝炎病毒阳性患者中也存在，在一些丙型肝炎病毒污染的患者中也存在 IGIV地段。丙型肝炎病毒与庚型肝炎病毒混合感染的临床意义 被评估。此外，由于IGIV的制造现在包括以下步骤 灭活或去除某些脂质包裹的病毒，目前尚不清楚 这些过程对HGV是否也有类似的作用。

Hepatitis G virus (HGV) is a recently identified RNA virus which appears to be transmitted by parenteral exposure to blood and blood products, and may be associated with clinical hepatitis in humans. The objective of this study was to evaluate the prevalence of HGV in HCV infected individuals and in HCV contaminated Immune Globuin Intravenous (IGIV) products. Different sets of nested primers, specific for the 5' non-coding region of HGV, were designed and tested in a reverse transcription and polymerase chain reaction (RT-PCR) assay. The set of primers that was found to be most effective in detecting HGV was used to test serum samples from chronically infected HCV positive (HCV 2.0 ELISA, RIBA 2.0 and HCV RNA positive) patients and 6 HCV RNA positive IGIV lots. HGV specificity of the PCR products was confirmed by sequencing and comparing them with the published sequence for HGV. HGV RNA was detected in 14 of the 50 (28%) HCV infected patient serum samples, and in 2 of the 6 (33%) IGIV lots. These results indicate that HGV RNA is quite prevalent among HCV positive patients and is also present in some HCV contaminated IGIV lots. The clinical significane of HCV and HGV co-infection needs to be evaluated. Moreover, since the manufacture of IGIV now includes steps to inactivate or remove certain lipid enveloped virus, it is not known whether these processes also act similarly on HGV.