MOLECULAR NOVELTY IN SEQUENCES OF BACTERIA AND MODEL ORGANISMS

细菌和模型生物序列中的分子新颖性

• 批准号: 2578625
• 负责人: J C WOOTTON
• 金额: --
• 依托单位: NATIONAL LIBRARY OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2578625
• 关键词: DNA binding protein; DNA repair; bacterial proteins; biochemical evolution; computer assisted sequence analysis; computer data analysis; computer system design /evaluation; microorganism metabolism; protein sequence; protein structure function

Protein sequences deduced from gene sequences of diverse bacteria land archaebacteria, and also, increasingly, from eukaryotic model organisms, are yielding a wealth of new knowledge on protein functions, interactions and evolution. Novel findings, that emerged initially from computational analysis of sequences and sequence databases, have been studied in collaboration with experimental laboratories by concerted methods including directed mutagenesis and spectroscopic/enzymological analyses. Computational strategies are being developed to recognize some of the functional amino acid sequence patterns involved, many of which are subtle and variable. A. Proteins and protein complexes involved in DNA binding, mutagenesis and repair. For patterns of wall-established DNA binding regions, new methods for evaluation of pattern discriminators are being developed and applied to more novel patterns. Low- complexity sequences are frequent in components of multisubunit DNA-binding complexes and require analysis and filtering before database searches. Methods of automated local multiple alignment by iterative sampling are included and evaluated based on test sets of various types of DNA-binding motifs. B. Sequence families in complex bacteria including pathogens. The rapidly growing body of new sequences from pathogenic bacteria, and widely diverse prokaryotes such as multicellular or differentiating bacteria, cyanobacteria and archaebacteria, were investigated by a range of computational analyses. More than 50 percent of the classifiable protein sequences did not have counterparts in E. coli and other well-studied bacteria, and many of these had eukaryotic homologs. Examples of low-complexity segments and multiple repeats are emerging with increasing frequency, especially in proteins involved in surface interactions, for example with the immune system, many of which evolve rapidly. In other cases, novel chemical and metabolic functions have been found. Genome sequences and protein structural studies from matabolically and morphogenetically diverse bacteria and other model organisms continue to provide a rich and cost-effective source of new discoveries on the molecular functions and evolution of proteins including aspects related to human diseases.

从不同的基因序列推导的蛋白质序列 细菌土地古细菌，而且，越来越多，从 真核模式生物，正在产生大量新的 了解蛋白质的功能、相互作用和进化。 新的发现，最初出现在计算 序列和序列数据库的分析，已经被 与实验室合作研究， 协同方法，包括定向诱变和 光谱/酶学分析。 计算 目前正在制定战略， 功能性氨基酸序列模式涉及，许多 微妙而多变 A. 参与DNA结合的蛋白质和蛋白质复合物， 诱变和修复。 对于墙的模式建立 DNA结合区，模式评价的新方法 鉴别器正在开发并应用于更多 新颖的模式。 低复杂度序列在 多亚基DNA结合复合物的组分， 在数据库搜索之前需要分析和过滤。 通过迭代的自动局部多重比对方法 包括抽样，并根据以下测试集进行评估： 各种类型的DNA结合基序。 B。 复杂细菌中的序列家族， 病原体 快速增长的新序列体， 病原菌和种类繁多的原核生物，如 多细胞或分化细菌，蓝细菌 和古细菌，进行了一系列的研究， 计算分析。 百分之50以上的普选 可分类的蛋白质序列在 E.大肠杆菌和其他研究充分的细菌，其中许多 有真核生物的同源物 低复杂度的例子 片段和多重重复序列的出现， 频率，特别是在蛋白质参与表面 例如，与免疫系统的相互作用， 它们进化得很快。 在其他情况下，新的化学品和 基因组序列和 蛋白质结构研究从代谢和 形态发生多样性细菌和其他模型 生物体继续提供丰富和具有成本效益的 新发现的分子功能的来源， 蛋白质的进化，包括与人类相关的方面 疾病