MODULATION OF PROTEIN KINASE ACTIVITY BY GENISTEIN IN PROSTATE CANCER

金雀花素对前列腺癌中蛋白激酶活性的调节

• 批准号: 2464560
• 负责人: R BERGAN
• 金额: --
• 依托单位: DIVISION OF CLINICAL SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2464560
• 关键词: antineoplastics; cell adhesion; chemoprevention; genistein; integrins; intermolecular interaction; neoplasm /cancer pharmacology; nutrition related tag; prostate neoplasms; protein tyrosine kinase; tissue /cell culture

Genistein is an isoflavinoid which is found in high amounts in soy. Its consumption has been associated with a low incidence of prostate cancer (PCa). This project sought to elucidate potential mechanisms by which genistein may have potential therapeutic effects upon prostate cancer. Our studies elucidated a potential antimetastatic effect by demonstrating that genistein increased the adhesion of PCa cells to solid matrix. Increased adhesion was shown to be time and concentration dependent, with adhesion related effects evident at genistein concentrations as low as 1 nM (with dietary consumption, average serum concentrations of free genistein are 10 nM). Associated with changes in cell morphology was the translocation of focal adhesion kinase (FAK; a tyrosine kinase) from the cytoplasm to membrane associated focal adhesion plaques (focal areas of cell adhesion to extracellular solid matrix). At the membrane, FAK formed a complex with beta-1-integrin (an important transmembrane cell adhesion molecule). The activity of FAK autokinase activity transiently increased during the process of cell adhesion, thereafter it decreased to below baseline levels.

染料木素是一种异黄酮，在大豆中含量很高。它的