IN SITU PREPARATION--POLYMER STATIONARY PHASES FOR HPLC

拟议研究的总体目标是制定程序 用于原位制备有效的聚合物填充柱， 高效液相色谱法（HPLC）。 在第一阶段， Fenris技术研究公司，已经确立了 它的方法是通过开发配方， 汉密尔顿PRP-1，一种广泛使用的商业聚合物， 柱，具有5微米直径的颗粒。 最低 Fernis塔的折合塔板高度小于 PRP-1 此外，在校正了稳态的差异之后， 相颗粒直径、与Fenris柱的背压 比汉密尔顿PRP-1低五倍以上。 在第二阶段，芬里斯技术研究公司，提出要进一步 探索配方和聚合调节的效果 关于固定相性能，目的是进一步 提高效率。 固定相的特征在于： 测定色谱效率，通过 扫描电子显微镜和测量表面积， 通过BET氮吸附法测定孔隙率。 另外在 将开发用于引入离子官能化的原位程序， 聚苯乙烯主链上的基团， 利用离子可以实现具有低背压高效率 交换以及反相色谱法。 建议的商业应用 该项目将导致低成本，有效的聚合物柱， 分析和制备高性能液体 层析

The overall goal of the proposed research is to develop procedures for in situ preparation of efficient polymer packed columns for high performance liquid chromatography (HPLC). In Phase I, Fenris Technology Research, Inc., has established the feasibility of its approach by devoloping formulations that are comparable in efficiency to Hamilton PRP-1, a widely used commercial polymer column with five micron diameter particles. The minimum reduced plate height for the Fernis column is smaller than for PRP-1. Furthermore, after correcting for difference in stationary phase particle diameter, the backpressure with the Fenris column is more than five times lower that with the Hamilton PRP-1. In phase II, Fenris Technology Research, Inc., proposes to further explore the effect of formulation and polymerization conditioning on stationary phase performance with the objective of further improving efficiency. Stationary phases will be characterized by determining chromatographic efficiency, examining morphology by scanning electron microscopy and measuring surface area and porosity by the BET nitrogen adsorption method. In addition, in situ procedures will be developed for introducing ionic functional groups onto the polystyrene backbone so that the advantages of high efficiency with low backpressure can be realized with ion exchange as well as reversed phase chromatography. PROPOSED COMMERCIAL APPLICATION This project will lead to low cost, effecient polymer columns for both analytical and preparative high performance liquid chromatography.