DNA SEQUENCES INVOLVED IN THE HEAVY CHAIN SWITCH

描述：拟议实验的总体目标是了解 调节开关重组的顺式作用元件。 研究人员已经建立了一种转基因小鼠模型，可以测量 从小鼠G1基因中适当地调节生殖系转录。他有 还准备了一系列可以进行转换的转基因小鼠 重组为G1。他将调查顺位代理的职能 通过进一步定位G1基因中的元件 转基因，通过分析它们与其他启动子相互作用的能力， 通过它们从内源性G1基因上的缺失以及通过研究 顺式作用元件对生殖系转录和基因突变的影响 开关重组。他还将研究基因转录调控。 G2a基因，强调g2a基因中的顺式作用元件和 重链3监管区域。

DESCRIPTION: The overall goal of the proposed experiments is to understand the cis-acting elements through which switch recombination is regulated. The investigator has established a transgenic mouse model that measures properly regulated germline transcription from the murine g1 gene. He has also prepared a line of transgenic mice that can undergo switch recombination to g1. He will investigate the function of the cis-acting elements in the g1 gene by further localization of the elements in transgenes, by analysis of their ability to interact with other promoters, by their deletion from the endogenous g1 gene and by studying the effect of mutations of the cis-acting elements on both germline transcription and switch recombination. He will also study the transcriptional regulation of the g2a gene, emphasizing both cis-acting elements in the g2a gene and in the heavy chain 3 regulatory region.