CORE-- BIOCHEMICAL MANAGEMENT

核心生物化学实验室将成为 整合本计划项目中提出的各项研究。 它 将为提交的样品提供一套统一的酶和免疫测定 每个子组件。 核心实验室也将成为 纯化的蛋白质和酶，包括钙调蛋白、钙调蛋白调节的 酶，蛋白激酶和磷酸酶，以及免疫试剂， 对个别调查的各种研究。 在提交给以下组织的所有样品中待分析的酶和蛋白质组 核心被选择来测试假设， 钙信号机制是细胞内的重要组成部分， 阿尔茨海默病的神经退化 所得数据的相关性 从对正常和AD人体组织进行的统一测定中， 直接假设。 这些结果与样品分析的比较 从实验动物和以各种方式处理的培养细胞中， 允许识别可能的因素（即，缺血性事件，β- 淀粉样蛋白的产生，糖皮质激素的过度产生），这可能会引发 Ca2+调节的生化改变导致神经元细胞死亡。

The Core Biochemistry Laboratory will be a major resource for the integration of the individual studies proposed in this Program Project. It will provide a uniform set of enzyme and immunoassays for samples submitted by each of the subcomponents. The Core Laboratory will also be a source of purified proteins and enzymes including calmodulin, calmodulin regulated enzymes, protein kinases and phosphatases, as well as immunoreagents for a variety of studies of individual investigations. The set of enzymes and proteins to be analyzed in all samples submitted to the Core was selected to test the hypothesis that alterations in the calcium signalling machinery are essential components in the neurodegeneration of Alzheimer's disease. Correlation of data obtained from uniform assays performed on normal and AD human tissues will test this hypothesis directly. Comparison of these results with analyses of samples from experimental animals and cultured cells treated in various ways, may permit the identification of possible factors (i.e., ischemic events, beta- amyloid production, glucocorticoid overproduction) which may trigger the biochemical alterations in Ca2+ regulation leading to neuronal cell death.