NEURONAL REORGANIZATION IN ADULT HYPERTENSIVE RATS

成年高血压大鼠的神经元重组

• 批准号: 6234138
• 负责人: J. Michael Wyss
• 金额: $ 17.71万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6234138
• 关键词: Alzheimer's disease; acetylcholine; central neural pathway /tract; cerebral cortex; dendrites; disease /disorder model; hypertension; intercellular connection; limbic system; neural degeneration; neural plasticity; neurotransmitter transport; renin angiotensin system; spontaneous hypertensive rat; synapses

Whereas many studies of the pathogenesis of Alzheimer's disease have focused on the neuronal losses that occur in the limbic cortex of these patients, the reorganization of the connections of the remaining neurons may play a significant role int he functional deficits of these patients. Our previous studies show that in aged rats (more than 24 months of age), neurons in the retrosplenial granular cortex alter their normal, mature structure and remodel their synaptic connections, likely in response to decreases in synaptic activity. Specifically,a the apical dendrites of layer II neurons withdraw from the outer half of layer I, where they are normally innervated by the axons of the anterior ventral thalamic nucleus. These dendrites subsequently arborize int he deeper half of this layer (an area that lacks thalamic innervation from the anterior ventral thalamic nucleus and an area in which t he apical dendrites of the layer II cells typically do not arborize). Further, in the aged rat, the apical dendrites of layer III neurons extend to the outer half of layer I (an area that they normally do not occupy) and appear to contract the terminals of thalamic axons in this region. Thus, in this area of the cortex the neurons in both layers II and III are engaged in dynamic reorganization in the aged rat. Subsequent studies have demonstrated that these changes occur in spontaneously hypertensive rats (SHR) but at a much earlier age (12 months); an age at which the rats are in relatively good health and can be run reliably in behavioral tests. The reorganization of the retrosplenial cortex ina the 12 month old SHR is correlated with a significant decrease in learning and memory. Further, recent findings demonstrate that the age- related plasticity of structure and function in the mature (12 months old) and even aged (more than 24 month old) SHR are prevented and/or significantly delayed by chronic antihypertensive therapy with an angiotensin converting enzyme inhibitor. Thus, the mature SHR offers an model that could significantly facilitate the study of the regulation of structure and function of limbic cortex neurons that are actively remodeling their structure and connections. The first specific aim will test the hypothesis that a local decrease in the release of acetylcholine into the retrosplenial cortex precedes (and contributes to) the age-related reorganization of neurons in this cortex. The second specific aim will test the hypothesis that an imbalance ina the brain renin-angiotensin system alters acetylcholine release in the retrosplenial cortex and thereby induces structural remodeling of the dendrites and connections in the mature SHR. The third specific aim will test the hypothesis that replacement of the cholinergic innervation of the retrosplenial cortex will delay/prevent the reorganization of layer I of the retrosplenial cortex in the aging shr. The fourth specific aim will test the hypothesis that the reorganization of the projection from the anterior thalamus to the limbic cortex leads to behavioral impairments int he mature SHR. Our general hypothesis is that the age-related remodeling of the retrosplenial cortex ina the mature SHR is the result of chronically decreased synaptic activation of the apical dendrites of the layer II neurons, i.e., use dependent plasticity.

然而，许多关于阿尔茨海默病发病机制的研究 集中在发生在边缘皮层的神经元损失， 患者，剩余神经元连接的重组 可能在这些患者的功能缺陷中起重要作用。 我们以前的研究表明，在老年大鼠（超过24个月的年龄）， 压后颗粒皮质中的神经元改变其正常的、成熟的 构造和重塑它们的突触连接，可能是为了响应 突触活动减少。 具体来说，a的顶端树突 第二层神经元从第一层的外半部分退出，它们在那里 通常由丘脑腹前核的轴突支配。 这些树突随后在该层的较深的一半中树枝状生长（即， 缺乏丘脑前腹侧神经支配的区域 核和一个区域，其中第二层细胞的顶端树突 通常不树枝化）。 此外，在老年大鼠中， 的第三层神经元延伸到第一层的外半部（一个区域， 通常不占据），并似乎收缩丘脑的终末 在这个区域。 因此，在皮层的这一区域， 第II层和第III层在老年大鼠中进行动态重组。 随后的研究表明，这些变化发生在 自发性高血压大鼠（SHR），但在更早的年龄（12 月）;大鼠处于相对良好的健康状态并且可以被 在行为测试中运行可靠。 压后肌的重组 12个月大的SHR的皮质中， 在学习和记忆方面。 此外，最近的研究结果表明，年龄- 成熟期（12月龄）结构和功能的相关可塑性 和甚至老年（超过24个月大）SHR被预防和/或 慢性抗高血压治疗显著延迟， 血管紧张素转换酶抑制剂。 因此，成熟的SHR提供了一个 该模型可以显着促进对监管的研究 边缘皮层神经元的结构和功能， 重塑它们的结构和联系 第一个具体目标将 检验乙酰胆碱释放的局部减少 进入压后皮质先于（并有助于）与年龄相关的 大脑皮层神经元的重组 第二个具体目标将 检验脑内肾素-血管紧张素失衡的假设 系统改变压后皮质中乙酰胆碱的释放， 诱导树突和连接的结构重塑， 成熟SHR。 第三个具体目标将检验以下假设： 替代压后皮质的胆碱能神经支配将 延迟/阻止压后皮质I层的重组， 老化的SHR.第四个具体目标将检验以下假设： 前丘脑到边缘系统的投射重组 皮质导致成熟SHR的行为障碍。 我们的一般 一种假说认为，与年龄相关的压后皮质重塑 在成熟SHR中， 激活 II层神经元的顶端树突，即，使用 依赖可塑性