NEURAL AND ENDOCRINE CONTROL OF THE ADRENAL

肾上腺的神经和内分泌控制

• 批准号: 2017213
• 负责人: THOMAS Joseph MCDONALD
• 金额: $ 15.31万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-21 至 2001-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2017213
• 关键词: adrenal glands; adrenocorticotropic hormone; bioassay; blood flow measurement; catecholamines; cell biology; corticosteroid receptors; cortisol; embryo /fetus hypoxia; glucocorticoids; high performance liquid chromatography; hormone receptor; hormone regulation /control mechanism; immunocytochemistry; in situ hybridization; molecular biology; neuroregulation; northern blottings; radioimmunoassay; sheep; spinal cord; splanchnic nerves; statistics /biometry; western blottings

DESCRIPTION: (Adapted from the applicant's abstract) The present study is designed to test the hypothesis that fetal splanchnic nerves are necessary for normal physiological fetal adrenocortical function in response to acute and chronic hypoxemia. This hypothesis will be tested utilizing fetal sheep with splanchnic nerve ablation and it is anticipated that adrenocortical responses will be blunted after splanchnic nerve section. Selected vascular, hormonal, cellular, and molecular responses of the fetal adrenal and sympathetic spinal cord will be quantified in response to an acute 60 minute (Aim 1) or a chronic 24 hour (Aim 2) hypoxemic challenge induced by infusion of nitrogen into the maternal trachea. Hormonal endpoints will be fetal cortisol, ACTH, AVP, PGE2, catecholamines, and maternal cortisol plasma concentrations. Molecular determinations will include immunocytochemical staining, in situ hybridization, and Northern and Western analysis of steroidogenic enzymes, CRH, c-fos, c-jun, PNMT, tyrosine hydroxylase, and glucocorticoid receptors in the fetal adrenal and sympathetic spinal cord.

描述：（改编自申请人的摘要）本研究是