C/EBP ALPHA AS MEDIATOR OF INSULIN ACTION IN ADIPOCYTES

描述(改编自申请人的摘要)：长期目标 这个项目的目的是为了加深对 转录因子C/EBPalpha对脂肪细胞基因表达的调控 新陈代谢。3T3-L分化过程中C/EBPalpha的诱导 前脂肪细胞分化为脂肪细胞并激活相关基因的表达 在创造和维持脂肪细胞表型，特别是胰岛素 反应性葡萄糖转运体GLUT-4，脂肪细胞脂结合蛋白 422/AP2和硬脂酰辅酶A去饱和酶1(SCD1)基因。 PI将测试胰岛素诱导的磷酸化变化的效果 C/EBPalpha活性的研究及C/EBPalpha在脑损伤中的作用 调节下游脂肪细胞基因的表达。

DESCRIPTION (Adapted from the applicant's abstract): The long-term goal of this project is to further the understanding of the role of the transcription factor C/EBPalpha in regulating adipocyte gene expression and metabolism. C/EBPalpha is induced during the differentiation of 3T3-L preadipocytes into adipocytes and activates the expression of genes involved in creating and maintaining the adipocyte phenotype, notably the insulin responsive glucose transporter Glut-4, adipocyte lipid binding protein 422/AP2, and stearoyl CoA desaturase 1 (SCD1) genes. The PI will test the effects of insulin-induced changes in phosphorylation on the activity of C/EBPalpha and elucidate the role of C/EBPalpha in regulating the expression of downstream adipocyte genes.