T SPECIFIC DNA BINDING PROPERTIES OF A HUMAN PROTEIN
人类蛋白质的特定 DNA 结合特性
基本信息
- 批准号:2734707
- 负责人:
- 金额:$ 20.87万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1991
- 资助国家:美国
- 起止时间:1991-08-01 至 2001-06-30
- 项目状态:已结题
- 来源:
- 关键词:DNA footprinting chemical binding complementary DNA cytokine receptors gene induction /repression genetic promoter element human genetic material tag interleukin 2 intermolecular interaction nonhistone nucleoprotein nucleosomes polymerase chain reaction site directed mutagenesis tissue /cell culture
项目摘要
DESCRIPTION: The HMG-I(Y) group of mammalian "high mobility group" (HMG)
chromatin proteins are in vivo regulators of gene expression and founding
members of a new class of nonhistone proteins called 'architectural
transcription factors'. The investigator has demonstrated in human T cells
that HMG-I(Y) proteins participate, along with transcription factors NF-kB,
Elf-1 and SRF, in the initial in vivo transcription induction of the
interleukin-2 receptor a-chain gene (IL-2Ra), an essential controlling step
in mounting an effective immune reaction. The goals of the proposed
research are to elucidate, at the molecular/mechanistic level, how different
regions of the HMG-I(Y) protein function in the transcriptional regulation
of the IL-2Ra gene promoter. The investigator has produced a large number
of both variant HMG-I(Y) proteins and mutant IL-2Ra promoter DNAs for use in
both in vitro and in vivo investigations of the protein-DNA complexes formed
on the IL-2Ra promoter during transcriptional activation. The Specific Aims
of the research are to use these mutant proteins and promoter DNAs to: 1)
Determine the relative importance of different peptide domains of the
HMG-I(Y) protein in the formation of a looped, stereospecific activation
complex on the promoter of the human IL-2Ra gene and investigate the
molecular events involved in such complex formation in vitro and in vivo.
2) Determine the effects of site-specific HMG-I(Y) protein phosphorylations
on protein-protein and protein-DNA interactions during promoter complex
formation in vitro and in vivo. 3) Investigate the role played by HMG-I(Y)
protein-induced alterations of the nucleosomal chromatin structure of the
IL-2Ra promoter region during gene transcriptional activation in vitro and
in vivo. A variety of extremely sensitive and quantitative biochemical,
biophysical, and biological techniques will be employed to analyze the
ability of mutant HMG-I(Y) proteins to participate in IL-2Ra promoter
complex formation and function. Results from these experiments will
contribute significant new information concerning the regulated expression
of the IL-2Ra gene, one of the rate limiting steps in T cell activation
during induction of the immune response.
描述:哺乳动物HMG- i (Y)组“高迁移率组”(HMG)
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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RAYMOND REEVES其他文献
RAYMOND REEVES的其他文献
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{{ truncateString('RAYMOND REEVES', 18)}}的其他基金
Role of HMGA1 proteins in DNA damage and excision repair
HMGA1 蛋白在 DNA 损伤和切除修复中的作用
- 批准号:
7025763 - 财政年份:2005
- 资助金额:
$ 20.87万 - 项目类别:
Role of HMGA1 proteins in DNA damage and excision repair
HMGA1 蛋白在 DNA 损伤和切除修复中的作用
- 批准号:
7390282 - 财政年份:2005
- 资助金额:
$ 20.87万 - 项目类别:
Role of HMGA1 proteins in DNA damage and excision repair
HMGA1 蛋白在 DNA 损伤和切除修复中的作用
- 批准号:
6926730 - 财政年份:2005
- 资助金额:
$ 20.87万 - 项目类别:
Role of HMGA1 proteins in DNA damage and excision repair
HMGA1 蛋白在 DNA 损伤和切除修复中的作用
- 批准号:
7191579 - 财政年份:2005
- 资助金额:
$ 20.87万 - 项目类别:
A.T-SPECIFIC DNA-BINDING PROPERTIES OF A HUMAN PROTEIN
人类蛋白质的 A.T 特异性 DNA 结合特性
- 批准号:
3305772 - 财政年份:1991
- 资助金额:
$ 20.87万 - 项目类别:
AT SPECIFIC DNA BINDING PROPERTIES OF A HUMAN PROTEIN
AT 人类蛋白质的特定 DNA 结合特性
- 批准号:
6018853 - 财政年份:1991
- 资助金额:
$ 20.87万 - 项目类别:
T SPECIFIC DNA BINDING PROPERTIES OF A HUMAN PROTEIN
人类蛋白质的特定 DNA 结合特性
- 批准号:
2396912 - 财政年份:1991
- 资助金额:
$ 20.87万 - 项目类别:
A.T-SPECIFIC DNA-BINDING PROPERTIES OF A HUMAN PROTEIN
人类蛋白质的 A.T 特异性 DNA 结合特性
- 批准号:
3305771 - 财政年份:1991
- 资助金额:
$ 20.87万 - 项目类别:
AT SPECIFIC DNA BINDING PROPERTIES OF A HUMAN PROTEIN
AT 人类蛋白质的特定 DNA 结合特性
- 批准号:
6179364 - 财政年份:1991
- 资助金额:
$ 20.87万 - 项目类别:
A.T-SPECIFIC DNA-BINDING PROPERTIES OF A HUMAN PROTEIN
人类蛋白质的 A.T 特异性 DNA 结合特性
- 批准号:
3305773 - 财政年份:1991
- 资助金额:
$ 20.87万 - 项目类别:
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- 批准号:
20H00373 - 财政年份:2020
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Grant-in-Aid for Scientific Research (A)














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