MUSCARINIC AND PEPTIDERGIC CONTROL OF EXOCYTOSIS

毒蕈碱和肽能对胞吐作用的控制

• 批准号: 2431252
• 负责人: ARUN R. WAKADE
• 金额: $ 21.64万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-05 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2431252
• 关键词: calcium channel; calcium flux; catecholamines; chromaffin cells; cyclic AMP; exocytosis; inositol phosphates; laboratory rat; membrane potentials; muscarinic receptor; neuropeptide receptor; nicotinic receptors; tissue /cell culture; vasoactive intestinal peptide; voltage /patch clamp; voltage gated channel

DESCRIPTION: (adapted from Applicant's Abstract) This application proposes to study the mechanism of muscarinic and peptidergic receptor mediated changes in electrical properties, intracellular calcium concentration, and secretion of catecholamines (CA). For this purpose chromaffin cells of adrenals derived from 4- to 5-week-old rats will be cultured. Patch clamp techniques will be used to measure changes in membrane potential and calcium current, Indo-1 fluorescence and laser photometry to quantitate intracellular calcium and electrochemical detection to monitor exocytotic secretion of CA. Laser photometry and electrochemistry will be combined to simultaneously monitor changes in intracellular calcium and exocytosis from the same cell on an identical, subsecond time scale.

描述：（改编自申请人的摘要）此申请 提议研究毒蕈碱和肽基受体的机制 介导的电性能变化，细胞内钙 浓度和分泌儿茶酚胺（CA）。 为此目的 衍生自4至5周大鼠的肾上腺染色体细胞将是 培养。 贴片夹技术将用于测量更改 膜电位和钙电流，Indo-1荧光和激光 光度法用于定量细胞内钙和电化学 检测以监测Ca的胞吐分泌。 激光光度法和 电化学将合并以同时监视 同一细胞上相同的，相同的细胞的细胞内钙和胞吐作用 超股时间尺度。