OVEREXPRESSION OF NERVE GROWTH FACTOR

描述：已知的神经营养因子不仅控制神经元存活， 但可能调节表型分化和成人神经病理性疼痛。在……里面 过量表达NT-3或NGF的转基因小鼠，感觉发生变化 神经元数目、投射和痛阈值。合作实验室拥有 判断成人感觉“综合表型”的能力 神经元，使用细胞内记录和标记。这些技术将 用于检测对照和转基因小鼠的单个感觉神经元 评估神经营养因子的过度表达是否会改变表型(目标1和 2)。还假设神经病理性疼痛会重演。 神经营养素依赖的过程。在目标3中，神经元的表型 负责神经病理性疼痛的人将被定性，而抗神经营养素 将使用抗血清来阻断交感/感觉神经元的形成。 被认为是调节神经病理性疼痛的连接。

DESCRIPTION: Neurotrophins are known to control not only neuronal survival, but may modulate phenotypic differentiation and adult neuropathic pain. In transgenic mice overexpressing NT-3 or NGF, changes are noted in sensory neuron number, projections and pain threshold. The collaborating lab has the ability to determine the "Comprehensive phenotype" of adult sensory neurons, using intracellular recording and labeling. These techniques will be used to examine individual sensory neurons of control and transgenic mice to assess if neurotrophin overexpression alters the phenotype (aims 1 and 2). It is also hypothesized that neuropathic pain recapitulates neurotrophin dependent processes. In aim 3, the phenotype of neurons responsible for neuropathic pain will be characterized, and antineurotrophin antisera will be used to block formation of the sympathetic/sensory neuron connections thought to mediate neuropathic pain.