PENTAPLEX CLONING VECTOR FOR DECAPLEX DNA SEQUENCING

用于 DECAPLEX DNA 测序的 PENTAPLEX 克隆载体

• 批准号: 2647442
• 负责人: DAVID Alan MEAD
• 金额: $ 10万
• 依托单位: LUCIGEN CORPORATION
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-15 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2647442
• 关键词: biotechnology; genetic markers; molecular cloning; nucleic acid sequence; transfection /expression vector

DESCRIPTION (Adapted from investigator's abstract): This is a new Phase I proposal seeking support to develop an new cloning vector for use in shotgun DNA sequencing strategies. It is argued that currently, shotgun DNA sequencing approaches, which dominate current high throughput efforts in the US, are in part limited by the requirement to produce enough templates to carry out the approximately 5-fold redundant sequencing of the human genome. The applicant proposes to develop a sequencing vector in which it may be possible to clone five DNA inserts simultaneously and to carry out bi-directional reads on each insert (i.e. 10 reads). This can reduce the number of template preparations required by up to 10 fold depending on exact strategy and the efficiency of cloning. It may be even possible to multiplex sequencing runs which could further reduce sequencing efforts downstream of the template preparation step. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述(改编自研究人员摘要)：这是一个新阶段 I建议寻求支持，开发一种新的克隆载体，用于 猎枪DNA测序策略。有人认为，目前，猎枪 主导当前高通量的DNA测序方法 在美国的努力，在一定程度上受到生产要求的限制 足够的模板来执行大约5倍的冗余 人类基因组的测序。申请人建议开发一种 一种可克隆五个DNA插入物的测序载体 同时在每个插入物上进行双向读取 (即10次读取)。这可以减少模板制备的数量 根据准确的策略和效率，最多可增加10倍 克隆技术。甚至有可能将多个测序运行 可以进一步减少模板下游的测序工作 准备步骤。 建议的商业应用：不可用