Expression Enhanced Natural Product Pathways Using Advanced Metagenomic Tools
使用先进的宏基因组工具表达增强的天然产物途径
基本信息
- 批准号:8903016
- 负责人:
- 金额:$ 35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-03-01 至 2017-02-28
- 项目状态:已结题
- 来源:
- 关键词:AddressAnabolismAnti-Bacterial AgentsAnti-Infective AgentsAntibioticsAntifungal AgentsBiological AssayBiological FactorsBuffaloesCenters for Disease Control and Prevention (U.S.)ChemicalsChemistryClinicalCloningCollectionComplexDNADataEngineeringEnvironmentEscherichia coliGene ExpressionGenesGeneticGenomicsGoalsGrowthHealthHigh-Throughput Nucleotide SequencingLeadLibrariesMarketingMeasurableMetagenomicsMethodsMicrobeMulti-Drug ResistanceNatural Product DrugOutcomeOutcomes ResearchPathway interactionsPatientsPersonal SatisfactionProceduresProcessProductionPropertyReportingSequence AnalysisSeriesSourceTechnologyTherapeuticTherapeutic AgentsTimeTranslationsViralantimicrobialbasecellular engineeringcostdesigndrug discoverydrug resistant bacteriaimprovedinnovative technologiesinterestlarge scale productionmicrobialnext generationnovelpathogenpublic health relevancescreeningsmall moleculesuccesstool
项目摘要
DESCRIPTION (provided by applicant): A post-antibiotic era of multiple drug resistance has begun to threaten the health and well-being of mankind. A crucial limitation in economically converting untapped natural product potential to final scalable production is the lack of next-generation tools to effectively access the full medicinal impact of natural environments. This project utilizes innovative technologies to capture entire small molecule pathways and express them for the first time in E. coli, resulting in a potent pipeline of novel environmentally-derived
therapeutic compounds. There are two unique components available to achieve this goal: 1) A new strain of engineered E. coli to support many of the unique building blocks needed for expression of natural product biosynthetic pathways and 2) A BAC library of 800 clones containing entire small molecule pathways up to 170 kb to be assayed for expression of anti-bacterial activities against the ESKAPE series of pathogens. The outcomes of this research are expected to significantly accelerate the discovery and production of novel antimicrobial compounds.
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
DAVID Alan MEAD其他文献
DAVID Alan MEAD的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('DAVID Alan MEAD', 18)}}的其他基金
High Fidelity Linear MicroVector to Clone Complex, Problematic, and Large DNAs
用于克隆复杂、有问题和大型 DNA 的高保真线性微载体
- 批准号:
9346284 - 财政年份:2017
- 资助金额:
$ 35万 - 项目类别:
Analytical Metagenomics Paradigm for Structure Based Screening
基于结构的筛选的分析宏基因组学范式
- 批准号:
8310684 - 财政年份:2012
- 资助金额:
$ 35万 - 项目类别:
Enabling Technologies for Low Resource Molecular Diagnostics
低资源分子诊断的支持技术
- 批准号:
8044040 - 财政年份:2010
- 资助金额:
$ 35万 - 项目类别:
Enabling Technologies for Low Resource Molecular Diagnostics
低资源分子诊断的支持技术
- 批准号:
7779226 - 财政年份:2010
- 资助金额:
$ 35万 - 项目类别:
NOVEL SOLUBILITY-ENHANCING PROTEIN EXPRESSION TECHNOLOGY
新型增强溶解度的蛋白质表达技术
- 批准号:
8501542 - 财政年份:2009
- 资助金额:
$ 35万 - 项目类别:
NOVEL SOLUBILITY-ENHANCING PROTEIN EXPRESSION TECHNOLOGY
新型增强溶解度的蛋白质表达技术
- 批准号:
8250969 - 财政年份:2009
- 资助金额:
$ 35万 - 项目类别:
Massively Parallel Single Cell Genomics of the Human Microbiome
人类微生物组的大规模并行单细胞基因组学
- 批准号:
7611525 - 财政年份:2008
- 资助金额:
$ 35万 - 项目类别:
HIGH THROUGHPUT CLONING OVEREXPRESSION AND PURIFICATION OF ACTIVE MEMBRANE PROT
活性膜保护的高通量克隆过表达和纯化
- 批准号:
7325336 - 财政年份:2007
- 资助金额:
$ 35万 - 项目类别:
Ex Cyto DNA Sequencing from Single Bacterial Colonies
单菌落的前细胞 DNA 测序
- 批准号:
7870519 - 财政年份:2006
- 资助金额:
$ 35万 - 项目类别:
Ex Cyto DNA Sequencing from Single Bacterial Colonies
单菌落的前细胞 DNA 测序
- 批准号:
7156326 - 财政年份:2006
- 资助金额:
$ 35万 - 项目类别:
相似海外基金
Bone-Adipose Interactions During Skeletal Anabolism
骨骼合成代谢过程中骨-脂肪相互作用
- 批准号:
10590611 - 财政年份:2022
- 资助金额:
$ 35万 - 项目类别:
Bone-Adipose Interactions During Skeletal Anabolism
骨骼合成代谢过程中的骨-脂肪相互作用
- 批准号:
10706006 - 财政年份:2022
- 资助金额:
$ 35万 - 项目类别:
Bone-Adipose Interactions During Skeletal Anabolism
骨骼合成代谢过程中骨-脂肪相互作用
- 批准号:
10368975 - 财政年份:2021
- 资助金额:
$ 35万 - 项目类别:
BCCMA: Foundational Research to Act Upon and Resist Conditions Unfavorable to Bone (FRACTURE CURB): Combined long-acting PTH and calcimimetics actions on skeletal anabolism
BCCMA:针对和抵抗不利于骨骼的条件的基础研究(遏制骨折):长效 PTH 和拟钙剂联合作用对骨骼合成代谢的作用
- 批准号:
10365254 - 财政年份:2021
- 资助金额:
$ 35万 - 项目类别:
Bone-Adipose Interactions During Skeletal Anabolism
骨骼合成代谢过程中骨-脂肪相互作用
- 批准号:
10202896 - 财政年份:2021
- 资助金额:
$ 35万 - 项目类别:
BCCMA: Foundational Research to Act Upon and Resist Conditions Unfavorable to Bone (FRACTURE CURB): Combined long-acting PTH and calcimimetics actions on skeletal anabolism
BCCMA:针对和抵抗不利于骨骼的条件的基础研究(遏制骨折):长效 PTH 和拟钙剂联合作用对骨骼合成代谢的作用
- 批准号:
10531570 - 财政年份:2021
- 资助金额:
$ 35万 - 项目类别:
Dissecting molecular mechanisms implicated in age- and osteoarthritis-related decline in anabolism in articular cartilage
剖析与年龄和骨关节炎相关的关节软骨合成代谢下降有关的分子机制
- 批准号:
10541847 - 财政年份:2019
- 资助金额:
$ 35万 - 项目类别:
Dissecting molecular mechanisms implicated in age- and osteoarthritis-related decline in anabolism in articular cartilage
剖析与年龄和骨关节炎相关的关节软骨合成代谢下降有关的分子机制
- 批准号:
10319573 - 财政年份:2019
- 资助金额:
$ 35万 - 项目类别:
Dissecting molecular mechanisms implicated in age- and osteoarthritis-related decline in anabolism in articular cartilage
剖析与年龄和骨关节炎相关的关节软骨合成代谢下降有关的分子机制
- 批准号:
10062790 - 财政年份:2019
- 资助金额:
$ 35万 - 项目类别:
Promotion of NAD+ anabolism to promote lifespan
促进NAD合成代谢以延长寿命
- 批准号:
DE170100628 - 财政年份:2017
- 资助金额:
$ 35万 - 项目类别:
Discovery Early Career Researcher Award