SENSITIVE TEST OF GENERALIZED STEATORRHEA

目前的"黄金标准"的定量评估脂肪， 72小时粪便脂肪，普遍推荐用于评估吸收不良 但由于其冗长且难以执行而大大未被充分利用。 我们提出了一种新的方法，直接测量从所有 原因，消除了72小时粪便试验的局限性， 保留其重要特征。我们的方法是基于管理 1，3--[13C]二硬脂基，2-油酰基甘油和不可吸收标记物 DyCI3与高脂肪餐，然后分析单次粪便。的 测试预计是敏感的脂肪消化不良和脂肪 酸吸收不良，成本有效，并提供高度 与个别患者的72小时粪便脂肪相关。在第一阶段，我们 通过显示Dy是 定量排泄，并遵循与13C相同的排泄动力学- 标记的甘油三酯。在第二阶段，我们会研究 方法导致临床使用的实际测试。我们将演示 标记的甘油三酯吸收具有重现性，准确反映了 个体患者的脂肪变性程度， 诊断敏感性/特异性。开发的检测试剂盒和分析 服务将通过BioChemAnalysis Corporation进行销售。 拟定商业应用： 这项研究将导致可用的测试工具包和程序 为了准确定量所有原因引起的脂肪酸， 只有一个凳子的收藏。因为这个过程 可重复性、准确性和使用相对简单， 用于临床诊断和研究。

The current "gold-standard" for quantitative assessment of steatorrhea, 72-h stool fat, is universally recommended in evaluation of malabsorption but vastly under-utilized because it is tedious and difficult to perform. We propose a novel approach for direct measurement of steatorrhea from all causes which eliminates the limitations of the 72-h stool test while retaining its important features. Our approach is based on administration of 1,3--[13C]distearyl, 2-oleolyl glycerol and the nonabsorbable marker DyCI3 with a high fat meal followed by analysis of a single stool. The test is expected to be sensitive to steatorrhea of maldigestion and fatty acid malabsorption, cost effective, and to provide results that are highly correlated with 72-h stool fat in individual patients. During Phase I, we demonstrated the feasibility of the approach by showing that Dy is quantitatively excreted and follows the same excretion kinetics as 13C- labeled triglyceride. During Phase II, we will study details of the approach leading to a practical test for clinical use. We will demonstrate that labeled triglyceride absorption is reproducible, accurately reflects the degree of steatorrhea in individual patients, and has excellent diagnostic sensitivity/specificity. The developed test kit and analytical services will be marketed through BioChemAnalysis Corporation. PROPOSED COMMERCIAL APPLICATION: This research will lead to the availability of a test kit and procedure for accurate quantitation of steatorrhea from all causes and requires collection of only a single stool. Because the procedure highly reproducible, accurate and relatively simple to use it will be applicable to clinical diagnostic use as well as use in research.