FIBER OPTIC, IN VIVO CONFOCAL MICROSCOPY

该项目的目标是设计、建造和测试光纤 用于获得人体共焦反射光图像的光学导管 活体上皮组织，近真实的几个微米分辨率 时间到了。该设备的预期应用是帮助 上皮性癌前病变的诊断和潜在筛查。至 展示光纤的潜在临床优势 共聚焦成像，我们将评估我们的设备的能力 测量体内组织形态以帮助识别 子宫颈和口腔粘膜癌前病变。我们相信 该项目的成功完成将提供一种临床工具 这可以极大地提高对 口腔粘膜、宫颈、尿路上皮癌前病变 膀胱、结肠和其他上皮性病变发生率较高的器官 癌症。我们已经设计并测试了这个装置的原型。 它使用单根光纤并且可以在市场上买到， 宏观光学元件。然而，要在以下位置实现图像 通过内窥镜获得接近视频速率的视频，这是一种小型多光纤设备 其中可以扫描整个上皮厚度是必需的。我们的 初步结果表明，这种装置是可行的。目标是 该方案的目的是设计、建造和测试三根多光纤 解决三大工程进步的原型 需要获得接近viedeo速率的整个上皮图像 具有微米分辨率的厚度。该项目的具体目标 TO：(1)设计了一种多光纤共焦系统(Prototype1)，该系统使用 商业上可用的宏观光学器件，用于从 75-150亩直径视场，横向分辨率2-3亩 从单个焦平面在1秒内实现5-7微米的轴向分辨率 与组织表面平行的深度，大约200亩 在组织表面下，(2)构建和测试该系统 利用模体，正常志愿者和正常志愿者的口腔粘膜 和癌前病变宫颈上皮在10例患者中的初步研究 宫颈上皮内瘤变(CN)，(3)研究宫颈上皮内瘤变的方法 活体对比度增强，(4)设计和测试1厘米外 用于商业用途的镜片的直径组件 原型1；然而，在原型2中，要成像的组织平面 不会与组织表面平行，而是会形成一小块 与曲面的角度，以便图像平面中的单元格范围 从上皮表面到基底膜， 以及(5)设计第三个原型，它将使用定制的光学元件 远端段的外径不大于3 Mm，可与标准内窥镜结合使用。 用于接触口腔粘膜和子宫以外部位的设备 宫颈。

The objective of this project is to design, construct and test a fiber optic catheter to obtain confocal reflected light images of human epithelial tissues in vivo with several micron resolution in near real time. The intended application of the device is to aid in the diagnosis, and potentially screening, of epithelial pre-cancers. To demonstrate the potential clinical advantages of fiber-optic confocal imaging, we will assess the capability of our device to measure tissue morphology in vivo to aid in the recognition of pre-cancers of the uterine cervix and oral mucosa. We believe that successful completion of this project will provide a clinical tool which could dramatically improve recognition and monitoring of epithelial precancers of the oral mucosa, uterine cervix, urinary bladder, colon, and other organs with high incidences of epithelial cancer. We have designed and tested a prototype of this device which uses a single optical fiber and commercially available, macroscopic optical components. However, to achieve images at near video rate through an endoscope, a small, multi-fiber device which can scan the entire epithelial thickness is required. Our preliminary results indicate that such a device is feasible. The goal of the proposal is to design, construct and test three multi-fiber prototypes to address the three major engineering advances required to achieve near viedeo rate images of the entire epithelial thickness with micron resolution. The specific aims of the project to: (1) design a multi-fiber confocal system (prototype1) which uses commercially available macroscopic optics to obtain images from a 75-150 mu diameter field of view with 2-3 mu lateral resolution and 5-7 mu axial resolution in 1 second from a single focal plane depth, parallel to the tissue surface, located approximately 200 mu beneath the tissue surface, (2) to construct, and test this system using phantoms, the oral mucosa of normal volunteers, and normal and pre-cancerous cervial epithelium in a pilot study of 10 patients ith cervical intra-epithelial neoplasia (CN), (3) to study methods of contrast enhancement in vivo, (4) to design and test a 1 cm outer diameter assembly of commercially available lenses to be used with prototype 1; however, in prototype 2, the tissue plane to be imaged will not be parallel to the tissue surface, but will form a small angle with the surface so that the cells in the image plane range from the surface of the epithelium to the basement membrane, and (5) to design a third prototype, which will use custom optics so that the outer diameter of the distal section is no greater than 3 mm and it can be used in conjuction with standard endoscopy equipment to access sites other than the oral mucosa and uterine cervix.