CYTOGENETIC ANALYSIS OF DISEASE VECTORING TICK SPECIES

拟议的研究将调查以下问题的性质和范围 新启动的长期稳定细胞中的染色体变异 不同种类扁虱的培养。扁虱是许多生物的主要媒介 影响人类及其家畜的病原体。滴答 细胞培养通常被用于各种研究中 扁虱的寄生虫学、病毒学和生理学 它们携带着；尽管对人类的遗传学普遍缺乏了解 培养的细胞以及遗传学如何影响研究。这个 拟议的研究将提供遗传学的初步调查 扁虱细胞的培养。重点将放在动态情况上 发生核型变异的地方；包括非整倍体变异， 在同一培养中的雄性/雌性细胞，以及来自杂交来源的细胞。全 这些变异中的一种以前在扁虱物种中被发现。 和/或细胞培养。细胞培养将提供一致的来源 应用和使用的高质量中期核型图 染色体显带技术的进一步发展。 细胞遗传学数据将在所有可用的estably tick上收集 细胞系和尽可能多的新启动的细胞系。种 将被调查的包括大多数北美扁虱物种，这些物种 已知是主要的疾病媒介。对每种文化的调查将 包括标准核型、C-带、C-带和银染 诺尔斯。其他技术，如G-、Q-和R-带，以及 将增加各种DNA碱基含量荧光显带技术 当它们变得可用时。这些技术的发展将是 由细胞培养提供的一致性与 在性腺南瓜上执行这些技术时遇到的问题 准备工作。这些数据将应用于人们知之甚少的 壁虱遗传学，并扩展到壁虱生物学，生理学，系统学， 和生物防治系统。

The proposed research will investigate the nature and extent of chromosomal variation in newly initiated and long-term, stable cell cultures of various tick species. Ticks are major vectors of many pathogen organisms which affect humans and their domestic animals. Tick cell cultures are routinely used in various investigations of the parasitology, virology, and physiology of ticks and the organisms that they carry; despite a general lack of knowledge on the genetics of the cultured cells and how the genetics could effect the investigations. The proposed research will provide the initial investigations of the genetics of tick cell cultures. Emphasis will be placed on dynamic situations where karyotypic variations occur; including aneuploid variants, male/female cells in the same culture, and cells of hybrid origin. All of these variations have been previously identified in tick species and/or cell cultures. Cell culture will provide a consistent source of quality metaphase karyotypic figures for use in the application and further development of chromosome banding techniques. Cytogenetic data will be collected on all of the available establish tick cell lines and as many newly initiated cell lines as possible. Species to be investigated include most species of North American ticks that are known to be major disease vectors. Investigations of each culture will include standard karyology, C-banding, C-banding and silver-staining for NORs. Additional techniques such as G-, Q-, and R-banding, along with various DNA base content fluorescent banding techniques, will be added as they become available. The development of these techniques will be facilitated by the consistency offered from cell culture versus the problems encountered with performing these techniques on gonadal squash preparations. The data will be applied to the poorly understood area of tick genetics and extended into tick biology, physiology, systematics, and biological control systems.