CYTOGENETIC ANALYSIS OF DISEASE VECTORING TICK SPECIES

疾病媒介蜱种的细胞遗传学分析

• 批准号: 3734178
• 负责人: SCOTT J GUNN
• 金额: --
• 依托单位: UNIVERSITY OF TEXAS RIO GRANDE VALLEY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3734178
• 关键词: Ixodes; animal population genetics; chromosomes; cytogenetics; disease vectors; genetic polymorphism; karyotype; species difference; statistics /biometry; ticks; tissue /cell culture

The proposed research will investigate the nature and extent of chromosomal variation in newly initiated and long-term, stable cell cultures of various tick species. Ticks are major vectors of many pathogen organisms which affect humans and their domestic animals. Tick cell cultures are routinely used in various investigations of the parasitology, virology, and physiology of ticks and the organisms that they carry; despite a general lack of knowledge on the genetics of the cultured cells and how the genetics could effect the investigations. The proposed research will provide the initial investigations of the genetics of tick cell cultures. Emphasis will be placed on dynamic situations where karyotypic variations occur; including aneuploid variants, male/female cells in the same culture, and cells of hybrid origin. All of these variations have been previously identified in tick species and/or cell cultures. Cell culture will provide a consistent source of quality metaphase karyotypic figures for use in the application and further development of chromosome banding techniques. Cytogenetic data will be collected on all of the available establish tick cell lines and as many newly initiated cell lines as possible. Species to be investigated include most species of North American ticks that are known to be major disease vectors. Investigations of each culture will include standard karyology, C-banding, C-banding and silver-staining for NORs. Additional techniques such as G-, Q-, and R-banding, along with various DNA base content fluorescent banding techniques, will be added as they become available. The development of these techniques will be facilitated by the consistency offered from cell culture versus the problems encountered with performing these techniques on gonadal squash preparations. The data will be applied to the poorly understood area of tick genetics and extended into tick biology, physiology, systematics, and biological control systems.

拟议的研究将调查的性质和程度 新启动和长期稳定细胞中的染色体变异 培养不同种类的蜱虫。 蜱虫是许多疾病的主要传播媒介 影响人类及其家畜的病原体。 蜱 细胞培养物通常用于各种研究， 寄生虫学、病毒学和蜱虫生理学以及 他们携带;尽管普遍缺乏对遗传学的知识， 培养的细胞以及遗传学如何影响研究。 的 拟议的研究将提供遗传学的初步调查， 蜱细胞培养物。 重点将放在动态情况 其中发生核型变异;包括非整倍体变体， 同一培养物中的雄性/雌性细胞，以及杂交起源的细胞。 所有 这些变异中的一种以前在蜱虫物种中被鉴定过 和/或细胞培养物。 细胞培养将提供一个一致的来源， 用于申请的高质量中期核型图， 染色体分带技术的进一步发展。 将收集所有可用的已建立蜱的细胞遗传学数据 细胞系和尽可能多的新启动的细胞系。 物种 包括大多数种类的北美蜱， 已知是主要的疾病媒介。 对每种文化的研究将 包括标准细胞核学、C-显带、C-显带和银染 NORs。 其他技术，如G-、Q-和R-显带，沿着 将增加各种DNA碱基含量荧光显带技术 当它们变得可用时。 这些技术的发展将是 由于细胞培养与细胞培养的一致性， 在性腺南瓜上进行这些技术时遇到的问题 准备工作 这些数据将被应用于知之甚少的领域， 蜱遗传学和扩展到蜱生物学，生理学，系统学， 生物控制系统。