GENOMIC ARRANGEMENT AND NUCLEOTIDE SEQUENCE OF SEX RELATED REPETITIVE DNA

该建议旨在阐明基因组排列， 基因组中与性别相关的重复DNA分子的核苷酸序列 家鸡和鹌鹑。这些研究是由 检查高度重复的DNA序列，通常称为卫星DNA， 在白色来航鸡里。因为大量的重复DNA 在雌性鸡中的比例比在雄性鸡中的比例高， 与性染色体相关的特定DNA序列。 在过去的两年里，主要的努力是针对 阐明性连锁DNA序列在rds中的作用。两种 两种鸟类的性别相关DNA序列（0.4kb和1.1kb）， 本研究分离纯化了弗吉尼亚Colinus virginianus和家鸡Gallus domesticutus 和表征了 竞争性DNA-DNA杂交、凝胶电泳和限制酶切 酶分析支持这些DNA序列的限制， 异配子性（雌性）。结构的详细描述 这些序列的基因组组织是目前研究的重点， 工作分离的0.4kb和1.0kb Xhol片段的克隆和测序 将是第一要务这些克隆体将被用于 筛选基因组印迹和基因组文库， 女性特有序列的整体结构。克隆的 并且凝胶纯化的片段可用于原位研究;然而， 鸡和鹌鹑序列之间同源性可以通过直接 序列比较

This proposal is aimed toward elucidating the genomic arrangement and nucleotide sequence of sex-related repetitive DNA molecules in the genome of the domestic chicken and bobwhite quail. These studies are initiated by examining the highly repetitive DNA sequences, often called satellite DNA, in the white leghorn chicken. Because of a greater amount of repetitive DNA in the female chicken than in the male, experiments were initiated to look for specific DNA sequences associated with the sex chromosomes. Over the last two years primary efforts have been directed toward elucidating the role of the sex linked DNA sequence in rds. Two kinds of sex-related DNA sequences (0.4kb and 1.1kb) from two species of birds, Colinus virginianus and Gallus domesticutus have been isolated, purified and characterized. Competitive DNA-DNA hybridization, gel electrophoresis and restriction enzyme analyses support the limitation of these DNA sequences to the heterogametic sex (female). A detailed characterization of the structure and genomic organization of these sequences is the focus of the current work. Cloning and sequencing eh 0.4kb and 1.0kb Xhol fragments isolated from gels will be the first priority. These clones will then be used to screen genomic blots and genomic libraries to derive a detailed picture of the overall organization of the female-specific sequences. Both the cloned and gel purified fragments can be used for the in situ studies; however, the homology between chick and quail sequences can be determined by direct sequence comparison.