STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF PLACENTAL LACTOGENS

胎盘泌乳素的结构和功能特征

基本信息

项目摘要

The mouse placenta produces two placental lactogens (PLs); mPL-I and mPL- II. The long term objectives of the project are to understand the functions of these hormones in various target tissues and to understand what regulates their production and concentrations in the fetal and maternal blood. Although relatively little is known about the biological activities and regulation of secretion of PLs in various species, it is clear that PLs participate in several important processes during pregnancy. Investigating the physiology of PLs will contribute to a better understanding of the processes that influence fetal health and survival; during pregnancy. These studies are a continuation of previous work in this laboratory on the biological and regulation of secretion of mPL-I and mPL-II. Several experiments include examining the regulation of secretion of proliferin (PLF) and PLF-related protein (PRP), which are proteins that are structurally similar to the PLs and are also produced by the mouse placenta. The specific aims of this project are: (1) The primary structure of calcyclin, a stimulator of mPL-II secretion will be determined. The protein will be localized in the conceptus by in situ hybridization, and the presence of binding sites for decidual calcyclin in the placenta will be assessed. Effects of decidual calcyclin on PL-I, PLF and PRP secretion will be examined in vivo. (2) Effects of epidermal growth factor, transforming growth factors alpha and beta, tumor necrosis factor-alpha, interleukin (IL)-beta, and IL-6 alone and in combinations, will be determined on giant cell differentiation and on mPL-I, mPL-II, PLF and PRP secretion by placental cells from various days of pregnancy. The sites of production of these agents in the conceptus will be determined by in situ hybridization. (3) The structure of the genes for mPL-I and mPL-II will be determined, and the promoter region will be examined for the presence of known response elements. Putative regulators of mPL-I and mPL-II expression identified in this way will be examined in vitro and in vivo for effects on mPL-I and mPL-II secretion. (4) A putative stimulator of mPL-I secretion produced by the pituitary gland will be characterized and if it appears to be novel, it will be purified and characterized. (5) An inhibitor of prolactin secretion produced by the placenta will be purified. Its interaction with mPL-I and mPL-II in regulating prolactin secretion will be examined in vitro. (6) A novel 29K insulin-like growth factor binding protein (IGBP) whose production is induced by mPLs will be purified and its primary structure will be determined. The liver will be examined for the presence of 29K IGFBP. The gestational profile of 29K IGFBP in liver and mammary gland will be determined. Regulation of 29K IGFBP by mPL-I and mPL-II in liver and mammary gland will be examined. (7) Proteins regulated by mPL-I and mPL-II in maternal liver will be identified.
小鼠胎盘产生两种胎盘催乳素:MPL-I和MPL-I。 II.项目的长期目标是了解 这些激素在不同靶组织中的作用并了解 是什么调节了它们在胎儿体内的产生和浓度? 母血。尽管人们对生物的了解相对较少 在不同物种中,pls的分泌活性和调节,它是 明确请参与几个重要的过程在 怀孕了。对PLS的生理学研究将有助于 更好地了解影响胎儿健康的过程和 存活;在怀孕期间。这些研究是先前研究的延续 在这个实验室中研究分泌物的生物学和调节 MPL-I和MPL-II。几项实验包括检查这项规定 增殖蛋白(PLF)和PLF相关蛋白(PRP)的分泌,这是 在结构上与pls相似的蛋白质,并且也能产生 被老鼠的胎盘。这项计划的具体目标是:(1) 刺激MPL-II分泌的钙周期蛋白的一级结构将是 下定决心。该蛋白将通过原位定位在胚胎中。 杂交和蜕膜钙周期蛋白结合位点的存在 在胎盘中的位置将被评估。蜕膜钙周期蛋白对PL-I的影响 体内将检测PLF和PRP的分泌。(2)表皮效应 生长因子、转化生长因子α和β与肿瘤坏死 因子-α、白介素β和白介素6单独或组合, 将取决于巨细胞分化和MPL-I,MPL-II, 妊娠不同天数胎盘细胞分泌的PLF和PRP。 概念说明书中这些制剂的生产地点将是 用原位杂交法测定。(3)基因的结构 将确定MPL-I和MPL-II,并将启动子区域 检查是否存在已知的响应元素。推定 以这种方式确定的MPL-I和MPL-II表达的调节因子将是 检测体外和体内对MPL-I和MPL-II分泌的影响。 (4)脑下垂体分泌MPL-I的假定刺激物 腺体将被特色化,如果它看起来是新的,它将是 提纯和鉴定。(5)催乳素分泌的抑制剂 由胎盘产生的物质将被提纯。它与MPL-I的相互作用 而MPL-II在调节催乳素分泌方面的作用将在体外进行检测。 (6)一种新型29K胰岛素样生长因子结合蛋白(IGBP),其 MPLS诱导的产物将被提纯,其一级结构 将会被确定。肝脏将被检查是否存在29K IGFBP。29K胰岛素样生长因子结合蛋白在肝脏和乳腺的妊娠分布 将会被确定。肝脏中MPL-I和MPL-II对29K IGFBP的调节作用 并进行乳腺检查。(7)受MPL-I和MPL-I调控的蛋白质 母体肝脏中的MPL-II将得到鉴定。

项目成果

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FRANK J. TALAMANTES其他文献

FRANK J. TALAMANTES的其他文献

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{{ truncateString('FRANK J. TALAMANTES', 18)}}的其他基金

STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF PLACENTAL LACTOGENS
胎盘泌乳素的结构和功能特征
  • 批准号:
    6430869
  • 财政年份:
    2001
  • 资助金额:
    $ 5.83万
  • 项目类别:
STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF PLACENTAL LACTOGENS
胎盘泌乳素的结构和功能特征
  • 批准号:
    6301811
  • 财政年份:
    2000
  • 资助金额:
    $ 5.83万
  • 项目类别:
STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF PLACENTAL LACTOGENS
胎盘泌乳素的结构和功能特征
  • 批准号:
    6107928
  • 财政年份:
    1999
  • 资助金额:
    $ 5.83万
  • 项目类别:
STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF PLACENTAL LACTOGENS
胎盘泌乳素的结构和功能特征
  • 批准号:
    6107189
  • 财政年份:
    1998
  • 资助金额:
    $ 5.83万
  • 项目类别:
PREGNANCY ASSOCIATED PROTECTION AGAINST BREAST CANCER
与怀孕相关的乳腺癌预防措施
  • 批准号:
    2456993
  • 财政年份:
    1996
  • 资助金额:
    $ 5.83万
  • 项目类别:
PREGNANCY ASSOCIATED PROTECTION AGAINST BREAST CANCER
与怀孕相关的乳腺癌预防措施
  • 批准号:
    2115174
  • 财政年份:
    1996
  • 资助金额:
    $ 5.83万
  • 项目类别:
PREGNANCY ASSOCIATED PROTECTION AGAINST BREAST CANCER
与怀孕相关的乳腺癌预防措施
  • 批准号:
    6602015
  • 财政年份:
    1996
  • 资助金额:
    $ 5.83万
  • 项目类别:
PREGNANCY ASSOCIATED PROTECTION AGAINST BREAST CANCER
与怀孕相关的乳腺癌预防措施
  • 批准号:
    2712807
  • 财政年份:
    1996
  • 资助金额:
    $ 5.83万
  • 项目类别:
PREGNANCY ASSOCIATED PROTECTION AGAINST BREAST CANCER
与怀孕相关的乳腺癌预防措施
  • 批准号:
    6500600
  • 财政年份:
    1996
  • 资助金额:
    $ 5.83万
  • 项目类别:
PREGNANCY ASSOCIATED PROTECTION AGAINST BREAST CANCER
与怀孕相关的乳腺癌预防措施
  • 批准号:
    2815959
  • 财政年份:
    1996
  • 资助金额:
    $ 5.83万
  • 项目类别:

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超生奥本海默化学中的化学结合理论及其在复杂分子系统中的应用
  • 批准号:
    20H00373
  • 财政年份:
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