NUTRIENT HOMEOSTASIS--COORDINATION OF RESPONSE BY THE NUCLEUS TRACTIS SOLITARIUS

营养稳态--孤束核反应的协调

• 批准号: 6240128
• 负责人: ROBIN A BARRACO
• 金额: $ 2.69万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240128
• 关键词: autonomic nervous system; catecholamines; cholecystokinin; deoxyglucose; galanin; glucose; homeostasis; insulin; laboratory rat; microinjections; neuroendocrine system; neuropeptide Y; norepinephrine; nutrition; pancreas hormone; perfusion; serotonin; solitary tract nucleus; somatostatin; starvation

The goal of this research is to determine the basic neuroreceptor signaling processes of nutrient homeostasis mediated by the nucleus of the solitary tract (NTS) for coordinating neural and humoral nutrient-related signals with the consequent production of appropriate autonomic and viscerobehavioral response patterns. Our general hypothesis is that the integration of nutrient-related information and the coordination of autonomic response patterns by the NTS occurs at both the afferent and efferent limbs of the neural organizational network mediating ingestive behavior, nutrient homeostasis and metabolic regulation. Our general hypothesis exploits the observation that the NTS is not only the principal recipient site in the CNS of first-order afferent input from the vast array of visceral sensory receptors involved in nutrient homeostasis, but the NTS also has extensive direct reciprocal connections with virtually all structures at every level of the neural axis involved in nutrient homeostasis, metabolic regulation and ingestive behavior. Thus, the specific aim of the proposed research is to determine the neuroreceptor signaling processes by which the NTS subserves the following multiple roles in nutrient homeostasis: (1) a chemosensory role for nutrient-related signals in the blood or CSF: (2) an integrative role for coordinating visceral sensations transmitted to the NTS mainly via the vagus nerve: (3) a major role as an autonomic control center in the brainstem for the production of "short-loop" homeostatic response patterns and (4) an effector role for some components of ingestive behavior. The overall research strategy employs two dimensions of experimental analysis which can be powerfully coordinated: (1) analysis at the tissue level - in vitro studies with the NTS slice preparation to examine the modulation of monoamine release by nutrient-related signaling substances including transmitters., hormones, nutrients and neuropeptides and (2) analysis at the physiological systems level - in vivo microinjection studies in the NTS to examine the modulation of viscerohumoral and autonomic response patterns elicited by application of nutrient-related signaling substances. These studies will provide an integrated, multidisciplinary spectrum of research experiences and research training opportunities in the neurosciences for MBRS student participants at both the undergraduate and graduate levels. Students will participate in all aspects of the research and contribute to an overall understanding of NTS-mediated mechanisms of nutrient homeostasis.

这项研究的目的是确定基本的神经受体信号传导 孤束核介导的营养稳态过程 协调神经和体液营养相关信号的神经束（NTS） 随之产生适当的自主神经， 内脏行为反应模式 我们的一般假设是， 营养素相关信息的整合和 NTS的自主反应模式发生在传入和 神经组织网络的传出支介导摄食 行为、营养平衡和代谢调节。 我们的一般 假设利用观察，NTS不仅是主要的 中枢神经系统中的受体位点，来自大量的 内脏感觉受体参与营养稳态，但NTS 也与几乎所有国家都有着广泛的直接互惠联系。 参与营养的神经轴的每一个层次的结构 体内平衡、代谢调节和摄食行为。 因此 这项研究的具体目的是确定神经受体 网络传输系统通过信令过程实现以下多种功能 在营养平衡中：（1）营养相关化学感受作用 血液或CSF中的信号：（2）协调的综合作用 内脏感觉主要通过迷走神经传递到NTS：（3） 作为脑干中的自主控制中心， 产生“短环”稳态反应模式和（4） 摄食行为某些成分的效应器作用。 整体 研究策略采用两个维度的实验分析， （1）组织水平的分析-体外 研究与NTS切片制备，以检查调制 营养相关信号物质释放单胺，包括 发射机。，激素、营养素和神经肽;（2）分析. 生理系统水平-NTS中的体内显微注射研究 检查内脏体液和自主神经反应模式的调制 由营养相关信号物质的应用引起。 这些 研究将提供一个综合的，多学科的研究范围 在神经科学方面的经验和研究培训机会， MBRS学生参与者在本科和研究生水平。 学生将参与研究的各个方面，并为 全面了解NTS介导的营养机制 体内平衡