NUTRIENT HOMEOSTASIS--COORDINATION OF RESPONSE BY THE NUCLEUS TRACTIS SOLITARIUS

这项研究的目的是确定基本的神经受体信号 孤束核介导的营养动态平衡过程 协调神经和体液营养相关信号的束(NTS) 随之而来的是适当的自主神经和 内脏行为反应模式。我们的总体假设是 与营养相关的信息的整合和协调 NTS的自主反应模式发生在传入和 调节进食的神经组织网络的传出肢体 行为、营养动态平衡和代谢调节。我们的将军 假说利用了观察到的NTS不仅是主要的 接收方站点在中枢神经系统的一阶传入输入来自浩瀚的数组 内脏感觉受体参与营养动态平衡，但NTS 也有广泛的直接相互联系，几乎所有 与营养有关的神经轴的每个水平的结构 动态平衡、代谢调节和进食行为。因此， 拟议的研究的具体目的是确定神经感受器 NTS充当以下多个角色的信令过程 在营养动态平衡中：(1)营养相关的化学感觉作用 血液或脑脊液中的信号：(2)协调的综合作用 内脏感觉主要通过迷走神经传递到NTS：(3) 作为脑干中自主控制中心的主要角色 产生“短环”动态平衡反应模式和(4)一种 摄食行为的某些组成部分的效应者角色。整体而言 研究策略采用了两个维度的实验分析，可以 强有力的协调：(1)组织层面的分析--体外 用NTS片剂研究其对脑电活动的调节作用 营养相关信号物质释放单胺，包括 递质、激素、营养素和神经肽以及(2)分析 NTS生理系统水平的体内微量注射研究 研究内脏-体液和自主神经反应模式的调节 通过应用与营养相关的信号物质而引发。这些 研究将提供综合的、多学科的研究范围。 在神经科学方面的经验和研究培训机会 本科生和研究生的MBRS学生参与者。 学生将参与研究的各个方面，并为 全面认识NTS的营养调控机制 动态平衡。

The goal of this research is to determine the basic neuroreceptor signaling processes of nutrient homeostasis mediated by the nucleus of the solitary tract (NTS) for coordinating neural and humoral nutrient-related signals with the consequent production of appropriate autonomic and viscerobehavioral response patterns. Our general hypothesis is that the integration of nutrient-related information and the coordination of autonomic response patterns by the NTS occurs at both the afferent and efferent limbs of the neural organizational network mediating ingestive behavior, nutrient homeostasis and metabolic regulation. Our general hypothesis exploits the observation that the NTS is not only the principal recipient site in the CNS of first-order afferent input from the vast array of visceral sensory receptors involved in nutrient homeostasis, but the NTS also has extensive direct reciprocal connections with virtually all structures at every level of the neural axis involved in nutrient homeostasis, metabolic regulation and ingestive behavior. Thus, the specific aim of the proposed research is to determine the neuroreceptor signaling processes by which the NTS subserves the following multiple roles in nutrient homeostasis: (1) a chemosensory role for nutrient-related signals in the blood or CSF: (2) an integrative role for coordinating visceral sensations transmitted to the NTS mainly via the vagus nerve: (3) a major role as an autonomic control center in the brainstem for the production of "short-loop" homeostatic response patterns and (4) an effector role for some components of ingestive behavior. The overall research strategy employs two dimensions of experimental analysis which can be powerfully coordinated: (1) analysis at the tissue level - in vitro studies with the NTS slice preparation to examine the modulation of monoamine release by nutrient-related signaling substances including transmitters., hormones, nutrients and neuropeptides and (2) analysis at the physiological systems level - in vivo microinjection studies in the NTS to examine the modulation of viscerohumoral and autonomic response patterns elicited by application of nutrient-related signaling substances. These studies will provide an integrated, multidisciplinary spectrum of research experiences and research training opportunities in the neurosciences for MBRS student participants at both the undergraduate and graduate levels. Students will participate in all aspects of the research and contribute to an overall understanding of NTS-mediated mechanisms of nutrient homeostasis.