GENETIC AND PHENOTYPIC VARIATION IN YEAST KILLER FACTOR

酵母杀伤因子是一种有价值的表型，用于工业、医疗、 和研究应用。它控制着商业发酵，艾滋病 在鉴定致病生物型方面，是一种 酵母菌感染的局部治疗。它最终可能会被用于 抗独特型和生物工程蛋白的生产。天然 致死因子毒素各不相同，这种变异增加了它们的 有用处。然而，人们对杀手的遗传基础知之甚少。 在许多杀手系统中产生毒素。这个项目将增加我们的 关于自然表型变异和杀手遗传学的知识 毒素形成。 为了实现这些目标，调查员将确定和定义 杀手毕希亚的杀手表型来自自然发生 坏死的仙人掌组织。该属由定义明确的种组成。 群体，其中许多包含杀手菌株，杀手表型将是 通过对每个杀手分离株进行筛选以确定其杀死一组 其他70个酵母菌。将为每种毒素确定物理最优 发现了变异。从这个数据库中，杀手毒素的遗传学 将通过不同的交配菌株来调查生产情况 每组相关毕赤酵母的表型。 学生们将学习如何鉴定酵母，进行基因分析，以及 组织和展示他们的数据。科学研究的跨学科问题 该项目将整合不同课程和援助中提出的概念 在发展量化和写作技能方面。这个 跨学科方法将拓宽学生在以下方面的视野 生物学，并因此鼓励他们拓宽他们的视野 选择生物学作为自己的职业。

Yeast killer factor is a valuable phenotype used in industrial, medical, and research applications. It controls commercial fermentations, aids in identifying pathogenic biotypes, and is the active components of a topical treatment for yeast infections. It may eventually be used in the production of anti-idiotypes and of bioengineered proteins. Natural killer factor toxins vary and this variation has increased their usefulness. However, little is known about the genetic basis of killer toxin production in many killer systems. This project will increase our knowledge of natural phenotypic variation and of the genetics of killer toxin formation. To accomplish these goals, the investigator will identify and define killer phenotypes from killer Pichia collected from naturally occurring necrotic cactus tissues. The genus is composed of well defined species groups, many of which contain killer strains, Killer phenotypes will be identified by screening each killer isolate for its ability to kill a set of 70 other yeast. Physical optima will be determined for each toxin variation identified. From this database, the genetics of killer toxin production will be investigated by mating strains with different phenotypes within each group of related Pichia. Students will learn to identify yeast, to do genetic analyses, and to organize and present their data. The interdisciplinary aspects of the project will integrate concepts presented in different courses and aid in developing both quantitative and writing skills. The interdisciplinary approach will widen the students' perspectives on biology and, consequently, encourage them to widen their perspectives on choosing a career in biology.