GENETIC AND PHENOTYPIC VARIATION IN YEAST KILLER FACTOR

酵母杀手因子的遗传和表型变异

• 批准号: 5211612
• 负责人: PHILIP F GANTER
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5211612
• 关键词: Ascomycetes; biological products; fungal genetics; genetic polymorphism; genetic strain; microorganism classification; microorganism metabolism; microorganism population study; microorganism toxin; phenotype; plant poisons; species difference; statistics /biometry; toxicant interaction

Yeast killer factor is a valuable phenotype used in industrial, medical, and research applications. It controls commercial fermentations, aids in identifying pathogenic biotypes, and is the active components of a topical treatment for yeast infections. It may eventually be used in the production of anti-idiotypes and of bioengineered proteins. Natural killer factor toxins vary and this variation has increased their usefulness. However, little is known about the genetic basis of killer toxin production in many killer systems. This project will increase our knowledge of natural phenotypic variation and of the genetics of killer toxin formation. To accomplish these goals, the investigator will identify and define killer phenotypes from killer Pichia collected from naturally occurring necrotic cactus tissues. The genus is composed of well defined species groups, many of which contain killer strains, Killer phenotypes will be identified by screening each killer isolate for its ability to kill a set of 70 other yeast. Physical optima will be determined for each toxin variation identified. From this database, the genetics of killer toxin production will be investigated by mating strains with different phenotypes within each group of related Pichia. Students will learn to identify yeast, to do genetic analyses, and to organize and present their data. The interdisciplinary aspects of the project will integrate concepts presented in different courses and aid in developing both quantitative and writing skills. The interdisciplinary approach will widen the students' perspectives on biology and, consequently, encourage them to widen their perspectives on choosing a career in biology.

酵母杀伤因子是一种有价值的表型，用于工业、医疗、 和研究应用。 它控制商业发酵，艾滋病 在鉴定病原生物型，是活性成分， 酵母菌感染的局部治疗。 它最终可能会被用于 抗独特型和生物工程蛋白质的生产。 自然 杀手因子毒素各不相同，这种变化增加了它们的 有用性 然而，对杀手的遗传基础知之甚少。 许多杀手系统中的毒素产生。 该项目将增加我们的 自然表型变异和杀手基因的知识 毒素形成 为了实现这些目标，研究者将确定和定义 从自然界中收集的杀手毕赤酵母的杀手表型 坏死的仙人掌组织 该属由明确定义的物种组成 组，其中许多含有杀手菌株，杀手表型将是 通过筛选每一个杀手分离物的能力来确定它是否能杀死一组 其他70种酵母 将确定每种毒素的物理最佳值 识别出变异。 从这个数据库中，致命毒素的基因 将通过使菌株与不同的 表型在每组相关的毕赤酵母中。 学生将学习识别酵母，做遗传分析， 整理和展示他们的数据。 的跨学科方面 项目将整合不同课程中提出的概念，并帮助 在发展定量和写作技能。 的 跨学科的方法将扩大学生的观点， 生物学，因此，鼓励他们扩大他们的观点， 选择生物学的职业