EFFECTS OF UV RADIATION ON UNICELLULAR ALGAE

紫外线辐射对单细胞藻类的影响

• 批准号: 6240071
• 负责人: ROBERT A KINZIE
• 金额: $ 1.52万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240071
• 关键词: algae; alternatives to animals in research; light adverse effect; photobiology; photosynthesis; photosystem; plant growth /development; species difference; ultraviolet radiation

The overall aim of this work is to investigate effects of current and predicted levels of long and middle range (UV-A, 320-400nm and UV-B, 280- 320 nm) ultraviolet radiation on photosynthetic systems, particularly phytoplankton and reef building corals. MBRS students have in the past and will continue to actively participate in several aspects of this work. The specific objectives of the MBRS student's research consist of training not only in proper use of the equipment, but more importantly in the ways of asking scientific questions and developing research projects to answer the questions so formulated. Specific research goals are 1) To elucidate effects of chronic exposure to UV-B on single celled algae, 2) To determine if there are species specific difference in tolerance to UV that might result in shifts in community composition under UV stress. The exposure experiments use two UV sources: 1) natural solar radiation filtered, when experimental protocol requires, through selective filters and 2) artificial radiation produced by our Xenon arc "solar simulator". The MBRS students receive training in quantification of light and UV radiation and in designing and building incubation systems. The effects of exposure are monitored through analysis of growth rates and composition of both photosynthetic and UV-absorbing pigments. Additionally photosynthetic capacity is measured before and after exposure to UV using 14C fixation. Effects of UV stress on species composition are initially being followed through two-species competition experiments run under differing levels of UV radiation.

这项工作的总体目标是调查电流和 预测水平的长期和中期（UV-A，320- 400 nm和UV-B，280- 400 nm） 320 nm）紫外线辐射对光合系统，特别是 浮游植物和造礁珊瑚。 MBRS学生在过去 并将继续积极参与其中的几个方面， 工作 MBRS学生研究的具体目标包括 培训不仅在正确使用设备，但更重要的是 在提出科学问题和开展研究的方式上， 项目，以回答这样制定的问题。 具体的研究目标是：1）阐明慢性暴露的影响 2）确定是否有物种 对紫外线耐受性的具体差异可能导致 UV胁迫下的群落组成。 暴露实验使用两种紫外线源：1）自然太阳辐射 当实验方案需要时，通过选择性过滤器过滤 2）由我们的氙弧“太阳模拟器”产生的人工辐射。 MBRS的学生接受光和紫外线定量的培训 辐射以及设计和建造孵化系统。 通过对生长率的分析来监测接触的影响 以及光合色素和紫外线吸收色素的组成。 此外，还测量了前后的光合能力 使用14 C固定暴露于UV。 紫外线胁迫对物种的影响 组成最初是通过两个物种的竞争 实验在不同水平的紫外线辐射下进行。