FGF SIGNALING IN BONE GROWTH AND DEVELOPMENT

FGF 信号在骨骼生长和发育中的作用

• 批准号: 2674131
• 负责人: David M Ornitz
• 金额: $ 21.54万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2674131
• 关键词: RNase protection assay; acetylcholine; biological signal transduction; bone development; cartilage development; chondrocytes; epiphysis; fibroblast growth factor; gene expression; genetic promoter element; genetically modified animals; growth factor receptors; immunocytochemistry; laboratory mouse; normal ossification; polymerase chain reaction; protein structure; tissue /cell culture

DESCRIPTION (adapted from investigator's abstract): This is a five year RO1 proposal to study the role of FGFR3 and FGF9 in bone development. The objectives of this proposal are to determine how FGFR3 regulates chondrogenesis and how it interacts with other signalling molecules thought to be important for endochondral ossification and to identify the physiological ligands that activate FGFR3 in vivo during endochondral ossification. The first aim will explore the expression of FGFR3 in the growth plates of normal mice using a transgenic mouse in which the LacZ gene is under control of the FGFR3 promoter and by immunohistochemistry. The second aim explores whether ligand affinity is altered by the Ach and TD mutation and whether chondrocyte gene activity is altered in culture. The third aim will ascertain by RT-PCR which FGFs are present in the adult growth plate. In the fourth aim it is proposed to complete a catalog of the expression of FGF9 by organ system ISH, RNAse protection assay, immunolocalization, FGF9-LacZ transgenic and the FGF9 gene structure will be completed.

描述（改编自研究者摘要）：这是一项为期5年的RO 1 建议研究FGFR 3和FGF 9在骨发育中的作用。 的 该提案的目的是确定FGFR 3如何调节 软骨形成以及它如何与其他信号分子相互作用， 对软骨内骨化很重要， 在软骨内分泌过程中体内激活FGFR 3的生理配体 骨化 第一个目的是探索FGFR 3在肿瘤细胞中的表达。 使用转基因小鼠的正常小鼠的生长板，其中LacZ基因 在FGFR 3启动子的控制下，并通过免疫组织化学。 的 第二个目的是探索配体亲和力是否被乙酰胆碱和TD改变 突变和软骨细胞基因活性是否在培养中改变。 的 第三个目标是通过RT-PCR确定哪些FGF存在于成人中 生长板 在第四个目标中，建议完成 通过器官系统ISH表达FGF 9，RNA酶保护测定， 免疫定位，FGF 9-LacZ转基因和FGF 9基因结构将被 完成