95F MYOSIN FUNCTION IN DROSOPHILA

这项建议的重点是确定一个在体内的功能 果蝇神经系统中的非常规肌球蛋白，95F肌球蛋白。95F 肌球蛋白的表达在整个生命周期中都受到发育调节 苍蝇的一些组织，包括神经系统，表达 相对较高的蛋白质水平。初步证据 证明95F肌球蛋白显示出很强的a定位 颗粒性损害中枢神经系统的神经细胞突起。 因为95F肌球蛋白已经被证明是一种马达 早期胚胎中的细胞质颗粒，颗粒染色 神经元可能表明95F肌球蛋白与颗粒有关，并可能 负责他们的运输。因此，我提议 探讨95F肌球蛋白在神经细胞发育中的作用 文化。我计划分析95F肌球蛋白介导的运动性和95F肌球蛋白 GFP-95F肌球蛋白融合后的体内分布。我还会检查 95F肌球蛋白突变对判断95F肌球蛋白是否 对神经元的发育和/或神经元内的运输很重要。 最后，L计划确定95F-肌球蛋白的性质 成熟神经元中的颗粒，并对95F肌球蛋白进行详细分析 蛋白质在胚胎神经系统的分布。建议数 实验将提供关于体内功能的新信息 95F肌球蛋白，以及基于肌动蛋白的转运机制 神经元。

The focus of this proposal is to determine the in vivo function of an unconventional myosin, 95F myosin, in the Drosophila nervous system. 95F myosin expression is developmentally regulated throughout the life cycle of the fly such that some tissues, including the nervous system, express comparatively higher levels of protein. Preliminary evidence demonstrates that 95F myosin exhibits strong localization of a particulate nature to nerve cell processes of the central nervous system. Because 95F myosin has been shown to be a motor that transports cytoplasmic particles in the early embryo, the particulate staining in neurons may indicate that 95F myosin is associated with particles and may be responsible for their transport. Therefore, I am proposing to investigate the in vivo function of 95F myosin in developing neurons in culture. I plan to analyze 95F myosin-mediated motility and 95F myosin distribution in vivo with a GFP-95F myosin fusion. I will also examine the effects of mutations in 95F myosin to determine whether 95F myosin is important for neuronal development and/or transport within neurons. Finally, l plan to determine the nature of 95F myosin-containing particles in mature neurons and perform detailed analysis of 95F myosin protein distribution in the embryonic nervous system. The proposed experiments will provide new information about the in vivo functions of 95F myosin, as well as the actin-based transportation mechanisms in neurons.