TRANSMITTER PHARMACOLOGY OF THE NEONATAL RAT SPINAL CORD

脊椎疼痛的处理机制在 出生后发育和成熟在吻部比尾部更早 级别。这增加了疼痛控制的药理学 新生儿的心弦可能与成人的有所不同。但很基本 关于脊柱发育药理学的研究很少。 和弦。后角是一个重要的研究场所，因为它代表着 脊椎疼痛传递(传入肽)和疼痛- 调制(球状脊髓单胺)系统首先收敛。给定 成熟的变化发生在吻尾，独立的分析 需要脊柱的吻部和尾部水平(相对于整个脊椎弦) 以避免对发育事件的错误解释。因此， 这些研究考察了疼痛处理的发育药理学。 脊索后角在颈椎和腰椎的作用机制 水平，并采用包括脊柱切片灌注在内的多种技术， RIA和高效液相色谱分析。具体目标是颈椎和腰椎 增大和：1)表征后角的成熟变化 初级传入物质P(SP)和降钙素的递质水平 基因相关肽(CGRP)、球茎脊髓去甲肾上腺素(NA)和 5-羟色胺(5-羟色胺)，2)检测这些神经递质的诱发释放 跨年龄，3)定义单胺受体系统的药理学 调节脊髓中P物质和降钙素基因相关肽的释放；4)发育研究 灯盏花素对单胺的功能性摄取和释放 终点站。这些研究将提供重要的新信息 脊柱疼痛处理的发育药理学及其比较 与成虫相比。

Spinal pain processing mechanisms undergo dramatic changes during postnatal development and mature earlier at rostral than caudal spinal levels. This raises the possibility that the pharmacology of pain control in the neonatal chord may differ from that of the adult. Yet basic research is scarce regarding the developmental pharmacology of the spinal chord. The dorsal horn is an important site for study as it represents the location where spinal pain-transmitting (afferent peptide) and pain- modulating (bulbospinal monoamine) systems first converge. Given the maturational changes proceed rostro-caudally, independent analyses of rostral and caudal spinal levels (versus whole spinal chord) are required to avoid erroneous interpretation of developmental events. Consequently, these studies examine the developmental pharmacology of pain processing mechanisms in the spinal chord dorsal horn at cervical and lumbar spinal levels and employ a number of techniques including spinal slice perfusion, RIA, and HPLC. The specific aims target the cervical and lumbar enlargements and: 1) characterize maturational changes in dorsal horn transmitter levels of primary afferent substance P (SP) and calcitonin gene-related peptide (CGRP), and bulbospinal noradrenaline (NA) and serotonin (5-HT), 2) examine the evoked release of these neurotransmitters across age, 3) define the pharmacology of monoamine receptor systems that modulate the spinal release of SP and CGRP, and 4) study the development of the functional uptake and release of monamines by bulbospinal terminals. These studies will provide important new information about the developmental pharmacology of spinal pain processing and how they compare to those of the adult.