NON NEURONAL MECHANISMS CONTRIBUTING TO STROKE PATHOLOGY

描述（申请人的摘要）：增加的血管渗透性是一种血管通透性增加的方法。 导致缺血性脑损伤的重要因素 和水肿。 这项研究的目的是检查缺氧对 血脑屏障的通透性特征。 第一个目标是 为了阐明在各种不同的刺激后BBB通透性的功能变化， 缺氧和模拟再灌注时间。 二是 确定钙依赖性蛋白的定位和表达， 细胞骨架蛋白、F-肌动蛋白和钙依赖性细胞粘附 分子，E-cadherin。 第三个目标是 内皮细胞核降解法研究内皮细胞凋亡 测量和检测BCL-2表达。 这项建议的重点是 非神经元机制导致中风的病理学。 研究 将提供关于可能有助于 观察缺血后的通透性变化。

DESCRIPTION (Applicant's Abstract): Increased vascular permeability is an important factor contributing to the development of ischemic brain damage and edema. The goal of this research is to examine the effect of hypoxia on permeability characteristics of the blood-brain barrier. The first Aim is to elucidate the functional changes in BBB permeability after various intervals of hypoxia and simulated reperfusion in vitro. The second is to determine the localization and expression of the calcium-dependent cytoskeletal protein, F-actin, and the calcium-dependent cellular adhesion molecule, E-cadherin, after hypoxic insults. The third Aim is to investigate endothelial cell apoptosis by performing nuclear degradation measurements and examining BCL-2 expression. The focus of this proposal is on non-neuronal mechanisms that contribute to stroke pathology. The studies will provide new information about mechanisms that may contribute to permeability changes observed after ischemia.