LONG TERM POTENTIATION OF HIPPOCAMPAL NEURONS

海马神经元的长期增强

• 批准号: 2832621
• 负责人: PAUL PAVLIDIS
• 金额: $ 3.15万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2832621
• 关键词: LONG; TERM; POTENTIATION; HIPPOCAMPAL; NEURONS

The long-range goal of the proposed research is to gain a detailed understanding of the cellular and molecular mechanisms underlying long- term potentiation (LTP) of synaptic transmission. Changes in the efficiency of transmission at synapses, such at LTP, are thought to be an important mechanism by which the function of the brain is modulated and by which memories might be stored. One of the most important and controversial issues surrounding LTP is whether its expression involves the presynaptic terminal. The proposed experiments provide critical specific and direct tests of the role of the presynaptic neuron in LTP, with an emphasis on determining the biochemical mechanisms involved. Some of the experiments are geared towards testing the role of the diffusible messenger nitric oxide (NO), which may act as a "retrograde messenger" from the postsynaptic neuron to the presynaptic neuron in LTP. LTP will be studied using electrophysiological recordings from pairs of synaptically coupled rat hippocampal neurons in organotypic culture. Pharmacological agents will be injected into presynaptic neurons so as to test their effects on LTP. The specific targets of the experiments include, but are not limited to, signal transduction pathways which might be activated by NO in presynaptic terminals.

拟议研究的远程目标是获得详细的 理解长长的细胞和分子机制 突触传递的术语增强（LTP）。变化 在LTP中，在LTP上的传播效率被认为是一种 调节大脑功能的重要机制 可以存储回忆。最重要的之一 LTP周围有争议的问题是其表达是否涉及 突触前末端。提出的实验提供了关键 特定和直接测试突触前神经元在LTP中的作用， 重点是确定所涉及的生化机制。一些 实验的旨在测试可扩散的作用 信使一氧化氮（NO）可以充当“逆行使者” 从突触后神经元到LTP的突触前神经元。 LTP将使用对成对的电生理记录进行研究 在器官培养中的突触耦合大鼠海马神经元。 药理剂将被注入突触前神经元，以便 测试其对LTP的影响。实验的特定目标 包括但不限于信号转导途径 在突触前末端中被NO激活。