SITE SPECIFIC REGULATION OF IMMUNE FUNCTION IN THE CNS

所提出的实验是基于这样的假设， 大鼠CNS的神经解剖区域将调节免疫细胞 不同的是在体内，这取决于局部的神经化学物质。 具体而言，细胞介导的免疫应答的两个主要方面， 在干扰素-γ）注射后增强- T细胞运输和MHC 表达-被认为是由局部中枢神经系统差异调节 环境.在这里，这些方面的免疫功能后， 注射部位：（1）孤束核 (NTS)（2）脑干区主要含有抑制性神经化学物质， 海马，主要含有兴奋性神经递质的区域，以及 (3)下丘脑，一个富含神经激素的区域。的 海马体和下丘脑的调节环境是 提出抑制T细胞运输和/或MHC表达，而 预计这些参数的增强将发生在NTS中。 针对特定神经化学物质的药理学拮抗剂将被 用于将免疫功能的差异调节归因于 神经传递素最后，诱导后的病变形成 实验性变态反应性脑脊髓炎 硬化症，将在这些神经解剖区域进行检查，以确定 是否神经递质的药理学操作会影响炎症。 拟议中的研究将增加对调节这些因素的深入了解。 对CNS中抗原的免疫应答。

The proposed experiments are based on the hypothesis that distinct neuroanatomical regions of the CNS of the rat will regulate immune cells differently in vivo, depending on the local profile of neurochemicals. Specifically, two major aspects of the cell-mediated immune response which are enhanced following interferon-gamma) injection - T cell traffic and MHC expression - are proposed to be differentially regulated by local CNS environments. Here, these aspects of immune function following INFO-gamma injection in the following regions: (1) the nucleus of the solitary tract (NTS), a brainstem region with primarily inhibitory neurochemicals, (2) the hippocampus, an area containing mainly excitatory neurotransmitters, and (3) the hypothalamus, a region with an abundance of neurohormones. The regulatory environments of the hippocampus and the hypothalamus are proposed to suppress T cell traffic and/or MHC expression, while enhancement of these parameters is expected to occur in the NTS. Pharmacological antagonists against specific neurochemicals will then be used to attribute differential regulation of immune function to major neurotransmitters. Finally, lesion formation after induction of experimental allergic encephalomyelitis, an animal model of multiple sclerosis, will be examined in these neuroanatomical regions to determine if pharmacological manipulation of neurotransmitters affects inflammation. The proposed studies will add insight into the factors that regulate the immune response to antigen in the CNS.