CYCLOOXYGENASE AND PC12 CELLS

已知环氧合酶在生理功能中具有重要作用，例如 炎症和疼痛调节。最近，一种诱导形式的这种 发现了环氧合酶2(COX-2)。环氧合酶-2的诱生作用 炎症介质，如白介素1b，以及已知的激活因子 蛋白激酶-C(即佛波酯，如TPA)，可能通过核因子)kB。 为了更好地了解中枢神经系统前列腺素的产生机制， 我们检测了原代星形胶质细胞中COX-2的活性和表达。 文化。我们展示了前列腺素的协同增加 11)1B和TPA的累积和COX-2活性，以及 假设这些药物是通过一种共同的途径起作用的 Nf)kB。

Cyclooxygenase is known to be important in physiologic functions such as inflammation and pain modulation. Recently, an inducible form of this enzyme, Cyclooxygenase)2 (COX-2),was discovered. COX-2 induced by inflammatory mediators such as IL)lB, as well as agents known to activate protein kinase-C (i.e. phorbol esters like TPA), possibly through NF)kB. To better understand the mechanisms of prostaglandlin production in the CNS, we have examined COX-2 activity and expression in primary astrocytes cultures. We demonstrate synergistic increase in prostaglandlin accumulation and COX-2 activity with the application of lL)1B and TPA, and postulate that these agents are working through a common pathway mediated by NF)kB.