CYCLOOXYGENASE AND PC12 CELLS

已知环氧合酶在生理功能中是重要的， 炎症和疼痛调节。 最近，一种诱导型的 环氧化酶）2（考克斯-2）。 诱导的考克斯-2 炎症介质如IL-1B，以及已知激活 蛋白激酶-C（即佛波酯如TPA），可能通过NF）kB。 为了更好地理解CNS中的甘草素产生机制， 我们检测了考克斯-2在原代星形胶质细胞中的活性和表达， cultures. 我们证明了协同增加的glandlin - 应用IL-1B和TPA的累积和考克斯-2活性，和 假设这些代理人是通过一个共同的途径介导的 NF）kB。

Cyclooxygenase is known to be important in physiologic functions such as inflammation and pain modulation. Recently, an inducible form of this enzyme, Cyclooxygenase)2 (COX-2),was discovered. COX-2 induced by inflammatory mediators such as IL)lB, as well as agents known to activate protein kinase-C (i.e. phorbol esters like TPA), possibly through NF)kB. To better understand the mechanisms of prostaglandlin production in the CNS, we have examined COX-2 activity and expression in primary astrocytes cultures. We demonstrate synergistic increase in prostaglandlin accumulation and COX-2 activity with the application of lL)1B and TPA, and postulate that these agents are working through a common pathway mediated by NF)kB.