STRUCTURAL BIOLOGY OF THE GLYCINE RECEPTOR

描述：这项提案的总体目标是结构 重组人A1甘氨酸受体氯通道的测定。 甘氨酸受体是配体门控通道大家族的一部分， 介导突触的信号转导。一个成员的结构 这个家族，乙酰胆碱受体，已经被研究了三年。 尺寸的电子显微镜和低分辨率结构是 但该序列尚未被映射到该结构中。 实验的目的是区分合理的拓扑模型 这一家族的通道通过标记半胱氨酸残基进入 甘氨酸受体与化学切割试剂。世界银行的一个主要目标 建议将纯化的全息受体及其 胞外，配体结合区。功能性甘氨酸受体组合物 可以从重组蛋白中重组出单亚基类型的 是在co-P.I的实验室中生产的，产量足够结晶 审判。

DESCRIPTION: The overall goal of this proposal is the structure determination of recombinant human a1 glycine receptor chloride channel. The glycine receptor is part of a large family of ligand-gated channels that mediate signal transduction at the synapse. The structure of one member of this family, the acetylcholine receptor, has been studied in three dimensions by electron microscopy and a low resolution structure is available, however the sequence has not been mapped into this structure. Experiments are aimed at distinguishing plausible topological models for this family of channels by labeling cysteine residues engineered into the glycine receptor with chemical cleavage reagents. A major goal of the proposal is to crystallize purified holoreceptor as well as its extracellular, ligand binding domain. Functional glycine receptor composed of a single subunit type can be reconstituted from recombinant protein, and is produced in the co-P.I's lab in large enough yields for crystallization trials.