STRUCTURAL BIOLOGY OF THE GLYCINE RECEPTOR

甘氨酸受体的结构生物学

• 批准号: 6019282
• 负责人: Robert O. Fox
• 金额: $ 18.12万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6019282
• 关键词: chemical binding; chemical cleavage; chloride channels; crystallization; fluorescent dye /probe; glycine receptors; membrane proteins; protein structure; recombinant proteins; structural biology

DESCRIPTION: The overall goal of this proposal is the structure determination of recombinant human a1 glycine receptor chloride channel. The glycine receptor is part of a large family of ligand-gated channels that mediate signal transduction at the synapse. The structure of one member of this family, the acetylcholine receptor, has been studied in three dimensions by electron microscopy and a low resolution structure is available, however the sequence has not been mapped into this structure. Experiments are aimed at distinguishing plausible topological models for this family of channels by labeling cysteine residues engineered into the glycine receptor with chemical cleavage reagents. A major goal of the proposal is to crystallize purified holoreceptor as well as its extracellular, ligand binding domain. Functional glycine receptor composed of a single subunit type can be reconstituted from recombinant protein, and is produced in the co-P.I's lab in large enough yields for crystallization trials.

描述：该提案的总体目标是结构 重组人α1甘氨酸受体氯离子通道的测定。 甘氨酸受体是配体门控通道大家族的一部分， 介导突触的信号转导。 一个成员的结构 这个家族，即乙酰胆碱受体，已在三个方面进行了研究 电子显微镜尺寸和低分辨率结构是 可用，但是序列尚未映射到此结构中。 实验旨在区分合理的拓扑模型 该通道家族通过标记半胱氨酸残基设计成 甘氨酸受体与化学裂解试剂。 该组织的一个主要目标是 提议是结晶纯化的全感受器及其 细胞外配体结合域。 功能性甘氨酸受体组成 单一亚基类型可以由重组蛋白重建，并且 在 co-P.I 的实验室中以足够大的产量进行结晶生产 试验。