NMR OF HIV REVERSE TRANSCRIPTASE PRIMERS

描述：在本申请中，建议进行研究以确定 与反密码子相关的一系列分子的溶液结构 用于合成HIV负链的tRNA3lys引物的环 用核磁共振检测DNA。PI将合成与该蛋白对应的RNA17-MERS 反密码子环结构的茎环。他将在内部替换 这一系列天然存在于同一部位的修饰核苷 它们出现在tRNA3lys中。这些修改对 茎环的结构、稳定性和RT结合将是 已评估。调查员还表示，他打算调查 RNA修饰对RNA-RNA相互作用的影响可能是 对于与互补的RNA模板的引物相互作用很重要。 传统的2D核磁共振方法以及新的同核和 异核3D核磁共振实验将被用来指定RNA共振 并得出结构信息。距离几何与分子 将使用动力学计算方法来转换核磁共振结构 将约束转换为已定义的3D结构。

DESCRIPTION: In this application, studies are proposed to determine the solution structures of a series of molecules related to the anticodon loop of the tRNA3lys primer for the synthesis of the minus strand of HIV DNA using NMR. The PI will synthesize RNA 17-mers corresponding to the stem-loop of the anticodon loop structure. He will substitute within this series naturally occurring modified nucleosides at the same site that they appear in tRNA3lys. The influence of these modifications on the structure, stability and RT binding of the stem loop will be evaluated. The investigator also states that he intends to investigate the effect of RNA modification on RNA-RNA interactions that may be important for primer interactions with complimentary RNA templates. Traditional 2D NMR methods along with newer homonuclear and heteronuclear 3D NMR experiments will be used to assign RNA resonances and to derive structural information. Distance geometry and molecular dynamics calculational methods will be used to convert NMR structural constraints into defined 3D structures.