RESPONSIVE POLYMER ENGINEERED PROTEIN CONJUGATES

在这个提案中，我们建议联合收割机（a）最新的蛋白质工程 用（B）刺激响应性聚合物组合物制备（c） 响应性聚合物-工程化蛋白质（RP-EP）缀合物具有独特的 行为和用途。 更具体地说，我们建议共轭 温度、pH或光敏低聚物通过其一端 终止于活性识别位点附近或活性识别位点内的特定位点 选择的蛋白质，然后施加温度、pH或光刺激， 端“（即，允许绑定）或“关闭”（即，要么减少绑定 亲和力，完全阻止结合，或排出先前结合的受体） 靶配体或细胞与蛋白质识别的结合 绝佳的价钱 这种蛋白质可以是抗体、酶、激素、整合素， 白蛋白、链霉抗生物素蛋白或任何其它识别蛋白。 蛋白质可以 抗体、酶、激素、整联蛋白、白蛋白、链霉亲和素， 或任何其他识别蛋白。 能够开启或关闭生物 分子水平上的识别过程应该会导致许多有趣的， 多样而独特的应用。 在本提案中，我们关注的是 识别过程，及其在亲和分离中的应用， 诊断和生物传感器。

In this proposal, we propose to combine (a) the latest protein engineering techniques with (b) stimuli-responsive polymer compositions to produce (c) responsive polymer-engineered protein (RP-EP) conjugates that have unique behaviors and uses. More specifically, we propose to conjugate temperature-, pH- or light-sensitive oligomers by one of their terminal ends to a specific site near or within the active recognition site of selected proteins, and then to apply a temperature, pH or light stimulus to tern " (i.e., allow binding) or "off" (i.e., either reduce the binding affinity, prevent binding altogether, or eject a previously-bound receptor) the binding of the target ligand or cell to the proteins's recognition site. The protein could be an antibody, an enzyme, a hormone, an integrin, albumin, streptavidin, or any other recognition protein. The protein could be an antibody, an enzyme, a hormone, an integrin, albumin, streptavidin, or any other recognition protein. The ability to turn on or off biological recognition processes on a molecular level should lead to many interesting, diverse and unique applications. In this proposal we focus on the affinity recognition process, and its applications in affinity separations, diagnostics and biosensors.