LUNG PRESERVATION FOR TRANSPLANTATION

移植时的肺保存

• 批准号: 2693320
• 负责人: George Alexander Patterson
• 金额: $ 26.49万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2693320
• 关键词: RNase protection assay; bioengineering /biomedical engineering; biotechnology; dogs; gene expression; gene therapy; homologous transplantation; immunocytochemistry; in situ hybridization; laboratory rat; liposomes; lung; lung ischemia /hypoxia; lung transplantation; neutrophil; nitric oxide; nitric oxide synthase; nonhuman therapy evaluation; prostaglandin endoperoxide synthase; reperfusion; tissue /cell culture; tissue /organ preservation; transfection; transfection /expression vector; western blottings

DESCRIPTION: Successful application of lung transplantation is hampered by inadequate lung preservation techniques and the frequent development of lung allograft reperfusion injury. This injury is mediated by neutrophils. Inhibition of neutrophil adherence by modulating levels of nitric oxide and prostaglandin metabolites reduces ischemia reperfusion injury in the lung, improves lung graft function, decreases morbidity and mortality and reduces costs. Transfer of genetic material to lung grafts is feasible under circumstances consistent with clinical lung transplantation. The first specific aim of this proposal is to determine ideal circumstances for efficient gene transfer to lung grafts. In Specific Aim II, neutrophil adherence and lung reperfusion injury will be reduced by modulation of nitric oxide and prostaglandin E2 and prostacyclin. This will be accomplished by augmentation of lung graft levels of genes encoding for inducible nitric oxide synthase and prostaglandin synthase. These studies will be conducted in a sequential series of in vitro rat and canine lung transplant models.

肺移植的成功应用受到以下因素的阻碍