GLUTAMINE IN GUT DYSFUNCTION AND BACTERIAL TRANSCYTOSIS

描述(改编自申请者的描述)：小肠 上皮细胞(肠上皮细胞)有非常高的能量需求，因为它们的 强烈的吸收和分泌功能和高细胞率 营业额。谷氨酰胺是肠细胞的主要能量来源，它 参与预防肠道萎缩，调节血液流动，以及 防止热休克引起的细胞死亡。目前的提案旨在 谷氨酰胺对肠上皮细胞作用的细胞机制 采用体外培养的Caco-2细胞模型。调查员将检查 谷氨酰胺从根尖供应时在保护过程中的作用 边上。大肠埃希菌移位及与肠道细胞相互作用的步骤 将通过结构和功能研究进行检验。钠泵活动 将与其他新陈代谢操作一起进行评估。超微结构 分析将特别强调紧密连接蛋白。 将在断奶兔回肠上进行平行研究，以 体外系统中的比较分析。

DESCRIPTION (Adapted from applicant's description): Small intestinal epithelial cells (enterocytes) have a very high energy demand due to their intensive absorptive and secretory functions and the high rate of cell turnover. Glutamine is the dominant energy source for enterocytes and it takes part in prevention of gut atrophy, regulation of blood flow, and protection against heat shock induced cell death. The current proposal aims at elucidating the cellular mechanisms of glutamine action on enterocytes using the CaCo-2 cell in vitro model. The investigator will examine the role of glutamine in the protective process when supplied from the apical side. Steps in translocation and interaction of E. Coli with enterocytes will be examined by structural and functional studies. Sodium pump activity will be evaluated along with other metabolic manipulations. Ultrastructural analysis will be performed with special emphasis on tight junction proteins. Parallel studies will be carried out in weanling rabbit ileal loops for comparative analysis in an in vitro system.