60 HZ EMF EFFECTS ON CAPACITATIVE CALCIUM ENTRY

一些流行病学研究表明， 暴露于工频电磁场（PFEMF）可能 某些肿瘤发展的危险因素。 这 然而，关联往往很弱，没有普遍接受的 解释这是如何发生的机制。 的长期目标 这项工作是为了确定PFEMF（60 Hz）是否会影响 那些调节细胞增殖的信号转导机制 从而增加肿瘤发生的可能性。 钙离子 这些调节途径的主要组成部分， 在文献中建议特定的钙调节系统， 容量性钙内流机制可能对 电磁场 一个似是而非的建议，为什么这可能是如此，特别是在 负责维持细胞更新的干细胞 例如上皮组织和造血组织的细胞群， 提出了 如果正确的话，这个模型可以帮助解释EMF如何 增加患癌症和白血病的风险。其具体目的是 建议是测试这个假设的第一部分，即，以确定 正弦PFEMF是否能调节Ca 2+的进入 通过细胞内的容性进入系统。 将进行研究 用Swiss 3 T3和SV 40转化的3 T3细胞，大鼠骨肉瘤 细胞（ROS 17/2.8）和正常人成纤维细胞。 的基本协议 将是激活这个系统，并确定是否60赫兹 EMF可以调节Ca 2+流过该系统的速率。 该系统的激活将通过耗尽 细胞内钙储存与促有丝分裂剂或肿瘤 启动子，毒胡萝卜素。 在治疗过程中，细胞将 暴露于毫特斯拉范围内的正弦60 Hz EMF， 使用气隙反应器在10至30 V/cm范围内的电场 该实验室开发的系统。 这样做的好处之一 系统的一个优点是，它允许由于磁效应之间的分辨率 场和那些由于感应电场。 胞浆游离钙 将通过记录来自 钙特异性，发光，发光蛋白，水母发光蛋白。

Some epidemiological studies suggest that residential and occupational exposure to power frequency electromagnetic fields (PFEMF) may be a risk factor for the development of certain neoplasms. This association is often weak, however, and there is no generally accepted mechanism to explain how this might occur. The long term goal of this work is to determine whether or not PFEMF (60 Hz) can affect those signal transduction mechanisms that regulate cellular proliferation and thus increase the likelihood of tumorigenesis. Calcium ions are major components of such regulatory pathways and there are suggestions in the literature that a particular calcium regulatory system, the ~capacitative~ calcium entry mechanism, might be sensitive to EMF. a plausible suggestion for why this might be so, especially in the case of stem cells responsible for maintaining renewal cell populations such as those of epithelial and hematopoietic tissues, is presented. If correct, this model could help explain how EMF might increase the risk of cancers and leukemias. The specific aim of this proposal is to test the first part of this hypothesis, i.e., to determine whether or not sinusoidal PFEMF can modulate the entry of Ca2+ through the capacitative entry system in cells. Studies will be done with Swiss 3T3 and SV40-transformed 3T3 cells, rat osteosarcoma cells (ROS 17/2.8) and normal human fibroblasts. The basic protocol will be to activate this system and determine whether or not 60 Hz EMF can modulate the rate at which Ca2+ flows through this system. Activation of this system will be accomplished by depleting the intracellular calcium store with mitogenic agents or the tumor promoter, thapsigargin. During the course of treatment, cells will be exposed to sinusoidal, 60 Hz EMF in the milliTesla range with induced electric field in the 10 to 30 V/cm range using an air gap reactor system developed in this laboratory. One of the advantages of this system is that it allows for resolution between effects due to magnetic fields and those due to induced electric fields. Cytosolic free calcium levels will be monitored in real time by recording light output from the calcium specific, luminescent, photoprotein, aequorin.