60 HZ EMF EFFECTS ON CAPACITATIVE CALCIUM ENTRY

一些流行病学研究表明，居住和职业 暴露在工频电磁场(PFEMF)中 某些肿瘤发生的危险因素。这 然而，联想往往很弱，而且没有被普遍接受的 解释这种情况如何发生的机制。的长期目标是 这项工作是为了确定PFEMF(60赫兹)是否会影响 那些调节细胞增殖的信号转导机制 从而增加肿瘤发生的可能性。钙离子是 这些调控途径的主要组成部分有 文献中的建议是，特定的钙调节系统， ~容量性~钙进入机制，可能对 电动势。这是一个合理的建议，解释了为什么会这样，特别是在 干细胞负责维持更新细胞的情况 例如上皮和造血组织的种群，是 呈上了。如果正确的话，这个模型可以帮助解释电动势如何 增加患癌症和白血病的风险。这样做的具体目的是 建议是检验这一假设的第一部分，即确定 正弦PFEMF是否能调节钙离子内流 通过细胞内的容量性进入系统。我们会进行研究 用瑞士3T3和SV40转化的3T3细胞，大鼠骨肉瘤 细胞(ROS 17/2.8)和正常人成纤维细胞。基本协议 将激活这个系统，并确定60赫兹是否 电动势可以调节钙离子流过这个系统的速度。 该系统的激活将通过耗尽 有丝分裂原或肿瘤的细胞内钙储存 促进剂，thapsigargin。在治疗过程中，细胞将被 暴露在毫微秒范围内的正弦60赫兹电磁场中 使用气隙电抗器的10到30 V/cm范围内的电场 在这个实验室里开发的系统。这样做的好处之一是 系统的特点是，它允许在磁效应之间进行分辨 电场和由感应电场引起的电场。胞浆游离钙 将通过记录来自 钙专一性，发光，光蛋白，水飞蓟素。

Some epidemiological studies suggest that residential and occupational exposure to power frequency electromagnetic fields (PFEMF) may be a risk factor for the development of certain neoplasms. This association is often weak, however, and there is no generally accepted mechanism to explain how this might occur. The long term goal of this work is to determine whether or not PFEMF (60 Hz) can affect those signal transduction mechanisms that regulate cellular proliferation and thus increase the likelihood of tumorigenesis. Calcium ions are major components of such regulatory pathways and there are suggestions in the literature that a particular calcium regulatory system, the ~capacitative~ calcium entry mechanism, might be sensitive to EMF. a plausible suggestion for why this might be so, especially in the case of stem cells responsible for maintaining renewal cell populations such as those of epithelial and hematopoietic tissues, is presented. If correct, this model could help explain how EMF might increase the risk of cancers and leukemias. The specific aim of this proposal is to test the first part of this hypothesis, i.e., to determine whether or not sinusoidal PFEMF can modulate the entry of Ca2+ through the capacitative entry system in cells. Studies will be done with Swiss 3T3 and SV40-transformed 3T3 cells, rat osteosarcoma cells (ROS 17/2.8) and normal human fibroblasts. The basic protocol will be to activate this system and determine whether or not 60 Hz EMF can modulate the rate at which Ca2+ flows through this system. Activation of this system will be accomplished by depleting the intracellular calcium store with mitogenic agents or the tumor promoter, thapsigargin. During the course of treatment, cells will be exposed to sinusoidal, 60 Hz EMF in the milliTesla range with induced electric field in the 10 to 30 V/cm range using an air gap reactor system developed in this laboratory. One of the advantages of this system is that it allows for resolution between effects due to magnetic fields and those due to induced electric fields. Cytosolic free calcium levels will be monitored in real time by recording light output from the calcium specific, luminescent, photoprotein, aequorin.