EMFS EFFECTS ON CALCIUM LEVELS IN EXCITABLE CELLS

流行病学研究表明，暴露在60赫兹下 电磁场(EMF)可能对神经有不良影响。 系统。然而，目前关于电磁场如何产生的信息很少 神经细胞可能会相互作用。我们研究的目标是提供数据 这将开始弥合这一差距。初步研究结果显示， 分离的肾上腺髓质嗜铬细胞，一种特征良好的细胞 神经型细胞培养模型，细胞内量增加 钙对大约1mT的磁通密度的响应。在这些 研究表明，细胞内钙水平是通过荧光视频监测的 在电磁场暴露期间对细胞进行成像。的整体ALM 建议的研究是继续使用荧光成像技术 用嗜铬细胞内钙离子来确定电磁场诱导的 钙响应作为几个电动势参数的函数。具体的 需要研究的场曝光参数有：1)磁通范围 引起反应的密度；2)引起电动势的频率 钙离子的变化：3)电磁场持续时间的关系 暴露与钙反应；4)单一与多重的影响 电磁场暴露对钙反应的影响；以及，5)瞬变对 钙的反应。从这些研究中得出的数据对于 帮助更严格地评估环境电磁场是否存在 学校、住宅、职业性电磁场暴露带来的人体健康风险 交通环境代表着一个复杂的混合领域 强度、曝光持续时间、频率和瞬变。

Epidemiological studies have suggested that exposure to 60-Hz electromagnetic fields (EMFs) may have adverse effects on the nervous system. However, there is currently little information regarding how EMFs and neural cells may interact. The goal of our research is to provide data that will begin to bridge this gap. Results of preliminary studies show tha isolated adrenal medullary chromaffin cells, a well-characterized cell culture model of neural-type cells, undergo an increase in intracellular calcium in response to a magnetic flux density of around 1 mT. In these studies, intracellular calcium level was monitored by fluorescence video imaging of the cells during EMF exposure. The overall alm of the proposed research is to continue to use fluorescence imaging of intracellular calcium in chromaffin cells to define the EMF-induced calcium response as a function of several EMF parameters. The specific field exposure parameters to be studied are: 1) the range of magnetic flux densities that elicit responses; 2) the frequencies of EMF that cause changes in calcium;: 3) the relationship between the duration of the EMF exposure and calcium responses; 4) the effect of single versus multiple EMF exposures on calcium responses; and, 5) the effect of transients on calcium responses. Data derived from these studies will be important for helping to evaluate more critically whether environmental EMFs present a human health risk since EMF exposures in school, residential, occupational and transportation environments represent a complex mixture of field intensities, exposure durations, frequencies and transients.