EMFS EFFECTS ON CALCIUM LEVELS IN EXCITABLE CELLS

流行病学研究表明，暴露于60赫兹 电磁场（EMF）可能对神经系统产生不良影响。 系统然而，目前关于电磁场如何 和神经细胞可以相互作用。我们研究的目的是提供数据 这将开始弥合这一差距。初步研究结果表明， 分离的肾上腺髓质嗜铬细胞，一种特征明确的细胞 神经型细胞的培养模型，经历细胞内 钙响应于约1 mT的磁通量密度。在这些 研究中，通过荧光视频监测细胞内钙水平 在EMF暴露期间对细胞进行成像。的总体alm 建议的研究是继续使用荧光成像， 嗜铬细胞内钙，以确定电磁场诱导的 钙响应作为几个EMF参数的函数。具体 研究的磁场暴露参数为：1）磁通量范围 密度引起的反应; 2）电磁场的频率，导致 钙的变化;：3）EMF持续时间之间的关系 暴露和钙反应; 4）单次与多次暴露的影响 EMF暴露对钙反应的影响;以及，5）瞬变对 钙反应。从这些研究中获得的数据对于以下方面很重要： 有助于更严格地评估环境电磁场是否存在 学校、住宅、职业场所电磁场暴露对人体健康危害 和运输环境代表了一个复杂的混合领域， 强度、暴露持续时间、频率和瞬变。

Epidemiological studies have suggested that exposure to 60-Hz electromagnetic fields (EMFs) may have adverse effects on the nervous system. However, there is currently little information regarding how EMFs and neural cells may interact. The goal of our research is to provide data that will begin to bridge this gap. Results of preliminary studies show tha isolated adrenal medullary chromaffin cells, a well-characterized cell culture model of neural-type cells, undergo an increase in intracellular calcium in response to a magnetic flux density of around 1 mT. In these studies, intracellular calcium level was monitored by fluorescence video imaging of the cells during EMF exposure. The overall alm of the proposed research is to continue to use fluorescence imaging of intracellular calcium in chromaffin cells to define the EMF-induced calcium response as a function of several EMF parameters. The specific field exposure parameters to be studied are: 1) the range of magnetic flux densities that elicit responses; 2) the frequencies of EMF that cause changes in calcium;: 3) the relationship between the duration of the EMF exposure and calcium responses; 4) the effect of single versus multiple EMF exposures on calcium responses; and, 5) the effect of transients on calcium responses. Data derived from these studies will be important for helping to evaluate more critically whether environmental EMFs present a human health risk since EMF exposures in school, residential, occupational and transportation environments represent a complex mixture of field intensities, exposure durations, frequencies and transients.