EMFS EFFECTS ON CALCIUM LEVELS IN EXCITABLE CELLS

EMFS 对兴奋细胞钙水平的影响

• 批准号: 2019323
• 负责人: GALE Louise CRAVISO
• 金额: $ 7.22万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-15 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2019323
• 关键词: calcium flux; calcium metabolism; chromaffin cells; electromagnetic radiation; magnetic field; radiobiology; tissue /cell culture; videotape /videodisc

Epidemiological studies have suggested that exposure to 60-Hz electromagnetic fields (EMFs) may have adverse effects on the nervous system. However, there is currently little information regarding how EMFs and neural cells may interact. The goal of our research is to provide data that will begin to bridge this gap. Results of preliminary studies show tha isolated adrenal medullary chromaffin cells, a well-characterized cell culture model of neural-type cells, undergo an increase in intracellular calcium in response to a magnetic flux density of around 1 mT. In these studies, intracellular calcium level was monitored by fluorescence video imaging of the cells during EMF exposure. The overall alm of the proposed research is to continue to use fluorescence imaging of intracellular calcium in chromaffin cells to define the EMF-induced calcium response as a function of several EMF parameters. The specific field exposure parameters to be studied are: 1) the range of magnetic flux densities that elicit responses; 2) the frequencies of EMF that cause changes in calcium;: 3) the relationship between the duration of the EMF exposure and calcium responses; 4) the effect of single versus multiple EMF exposures on calcium responses; and, 5) the effect of transients on calcium responses. Data derived from these studies will be important for helping to evaluate more critically whether environmental EMFs present a human health risk since EMF exposures in school, residential, occupational and transportation environments represent a complex mixture of field intensities, exposure durations, frequencies and transients.

流行病学研究表明，暴露于 60 Hz 电磁场（EMF）可能对神经产生不利影响 系统。然而，目前关于 EMF 如何作用的信息很少。 和神经细胞可能相互作用。我们研究的目的是提供数据 这将开始弥合这一差距。初步研究结果表明 分离的肾上腺髓质嗜铬细胞，一种特征明确的细胞 神经型细胞的培养模型，经历细胞内增加 钙对约 1 mT 磁通密度的响应。在这些 研究，通过荧光视频监测细胞内钙水平 EMF 暴露期间细胞的成像。的总体施舍 拟议的研究是继续使用荧光成像 嗜铬细胞中的细胞内钙来定义 EMF 诱导的 钙响应是多个 EMF 参数的函数。具体的 需要研究的场暴露参数有： 1）磁通量的范围 引起反应的密度； 2）引起的电动势的频率 钙的变化；：3）EMF持续时间之间的关系 暴露和钙反应； 4）单个与多个的效果 EMF 暴露对钙反应的影响；以及，5) 瞬态对的影响 钙反应。从这些研究中获得的数据对于 帮助更批判性地评估环境电磁场是否存在 学校、住宅、职业场所暴露于 EMF 后对人类健康造成的风险 和交通环境代表了复杂的现场混合体 强度、暴露持续时间、频率和瞬态。