BACKCROSS STRATEGY TO IDENTIFY ALCOHOL RELATED B6 QTLS
识别酒精相关 B6 QTLS 的回交策略
基本信息
- 批准号:2667595
- 负责人:
- 金额:$ 19.57万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-03-01 至 2001-02-28
- 项目状态:已结题
- 来源:
- 关键词:Mus musculus alcoholism /alcohol abuse alleles animal breeding animal genetic material tag behavior test behavioral /social science research tag behavioral genetics genetic mapping genetic markers genetic transcription genome genotype linkage mapping neurochemistry nucleic acid sequence phenotype polymerase chain reaction preference single strand conformation polymorphism
项目摘要
We propose the use of an intercross-backcross breeding protocol with
selective genotyping to identify, map, and characterize the genes
responsible for the high alcohol preference trait expressed by C57BL/6
mice. In our studies to date, we have identified and localized a
previously-unpredicted alcohol preference locus - Alcp1 - at a high level
of significance (P <0.00018) to an 8 cM interval on one mouse chromosome.
A second alcohol preference locus - Alcp2 - has been tentatively localized
on a second chromosome (P <0.0013). Both of these loci map close to genes
involved in serotonin activity. The specific aims of this research
proposal are: 1. Identification of loci involved in the alcohol preference
trait expressed by B6 mice. Additional offspring from the (B6 X DBA) X B6
backcross will be screened to obtain a total set of 300 animals that
express the high alcohol preference trait. A stratified analysis of these
animals with sets of microsatellite markers will allow us to identify and
map QTLs showing an association of at least 6O% with the alcohol
preferring class. Correlations among multiple QTLs will be defined and
used to evaluate various models of genetic interactions. A second
backcross will be analyzed to assess the generality of the results
obtained with B6 and DBA. 2. Genetic and molecular analysis of candidate
alcohol preference genes. Candidate serotonin-related genes already
identified, and any others suggested by the mapping studies of specific
aim 1, will be tested for co-segregation with Alcp1, Alcp2 , or further
Alcp loci that are uncovered. Candidate genes that survive this genetic
test will be assayed for coding sequence and RNA transcriptional
differences between parental B6 and DBA alleles. 3. Further neurochemical
and behavioral studies of alcohol-preferring mice. In collaboration with
Professor Bart Hoebel, alcohol-preferring mice will be analyzed for the
expression of additional addictive behavior traits and for specific
neurochemicals predicted to play a role in these traits based on the
genetic results of aims 1 and 2. If additional associations of any kind
are observed, the accumulated data will be used to determine the
specificity of genotype-phenotype relationships.
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项目成果
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LEE M SILVER其他文献
LEE M SILVER的其他文献
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{{ truncateString('LEE M SILVER', 18)}}的其他基金
BACKCROSS STRATEGY TO IDENTIFY ALCOHOL RELATED B6 QTLS
识别酒精相关 B6 QTLS 的回交策略
- 批准号:
2882038 - 财政年份:1996
- 资助金额:
$ 19.57万 - 项目类别:
BACKCROSS STRATEGY TO IDENTIFY ALCOHOL RELATED B6 QTLS
识别酒精相关 B6 QTLS 的回交策略
- 批准号:
2376088 - 财政年份:1996
- 资助金额:
$ 19.57万 - 项目类别:
BACKCROSS STRATEGY TO IDENTIFY ALCOHOL RELATED B6 QTLS
识别酒精相关 B6 QTLS 的回交策略
- 批准号:
6163744 - 财政年份:1996
- 资助金额:
$ 19.57万 - 项目类别:
BACKCROSS STRATEGY TO IDENTIFY ALCOHOL RELATED B6 QTLS
识别酒精相关 B6 QTLS 的回交策略
- 批准号:
2047641 - 财政年份:1996
- 资助金额:
$ 19.57万 - 项目类别:
CONTROL OF GENOMIC IMPRINTING BY A MOUSE IMPRINTOR LOCUS
通过小鼠印记基因座控制基因组印记
- 批准号:
2186641 - 财政年份:1993
- 资助金额:
$ 19.57万 - 项目类别:
CONTROL OF GENOMIC IMPRINTING BY A MOUSE IMPRINTOR LOCUS
通过小鼠印记基因座控制基因组印记
- 批准号:
3308437 - 财政年份:1993
- 资助金额:
$ 19.57万 - 项目类别:
CONTROL OF GENOMIC IMPRINTING BY A MOUSE IMPRINTOR LOCUS
通过小鼠印记基因座控制基因组印记
- 批准号:
2186640 - 财政年份:1993
- 资助金额:
$ 19.57万 - 项目类别: