CELL DIVISION GENE PRODUCTS OF BACILLUS SUBTILIS

描述：细菌细胞分裂是一个复杂的，高度调节的过程 其中细胞被膜组分的生物合成被重新定向以形成 分裂隔膜。 这一过程在时间和空间上都受到调节， 为了确保对蜂窝组件的均等划分， 细菌染色体 隔膜的形成必须涉及协调合成 细胞壁和细胞膜的组成部分。 的 细菌细胞分隔位点的化学性质仍然是最重要的化学性质之一。 在原核生物学中仍有一些重要的未解之谜。 的 细菌细胞分裂装置的高度保守性使得 这是未来抗菌药物开发的一个可能目标 化疗剂。 该项目涉及两种微细胞的分子遗传学特征， 决定因素（minC和divlVA）和三个细胞形状决定因素（mreBCD）， 革兰氏阳性细菌枯草芽孢杆菌。 这些药物的作用部位 细菌细胞中的蛋白质将通过免疫荧光检测 技术. 小细胞基因编码的蛋白质是已知最早的 参与细菌细胞分裂过程的作用蛋白质。 因此，它们的目标是在大部分细胞分裂之前的新生分隔部位。 细胞分裂蛋白参与了这一过程。 位点 小细胞相关蛋白，它们参与了 将鉴定到分隔位点的蛋白质。 DivlVA蛋白 已知参与了这一过程中的内生孢子形成 细菌，一个涉及不对称分隔事件的过程。 的 DivlVA细胞分裂蛋白和细胞分裂蛋白之间的界面的性质 将检查细菌孢子形成机制。 mre细胞形态 决定基因编码的蛋白质影响细胞形态， 参与细胞分裂过程。 MreC蛋白已经被 在指数生长的细胞中， 的B。枯草杆菌。 MreB、MreC和MreD蛋白在细胞凋亡中的作用 将研究划分过程。 通过分离抑制因子 突变，将鉴定与Mre蛋白相互作用的蛋白。

DESCRIPTION: Bacterial cell division is a complex, highly regulated process in which biosynthesis of the cell envelope components is reoriented to form a division septum. The process is regulated both temporally and spatially, to ensure equal partitioning of cellular components including a copy of the bacterial chromosome. Septum formation must involve coordinated synthesis of cell wall and cell membrane components of the cell envelope. The chemical nature of the bacterial cell septation site remains one of the most significant unanswered questions remaining in procaryotic biology. The highly conserved nature of the bacterial cell division apparatus makes this process a likely target for the development of future antibacterial chemotherapeutic agents. The project involves a molecular genetic characterization of two minicell determinants (minC and divlVA) and three cell shape determinants (mreBCD) of the Gram-positive bacterium Bacillus subtilis. The sites of action of these proteins in the bacterial cell will be examined by immunofluorescence techniques. The minicell genes encode proteins which are the earliest known acting proteins involved in the process of bacterial cell division. Therefore, they target the nascent septation site before the bulk of the cell division proteins become involved in the process. Sites on the minicelling-associated proteins which are involved in the localization of the proteins to the septation site will be identified. The DivlVA protein is known to be involved in the process of endospore formation in this bacterium, a process which involves an asymmetric septation event. The nature of the interface between the DivlVA cell division protein and the bacterial sporulation machinery will be examined. The mre cell shape determination genes encode proteins which affect cell morphology through their involvement in the cell division process. The MreC protein has been shown to be localized at the division septa in exponentially growing cells of B. subtilis. The roles of the MreB, MreC, and MreD proteins in the division process will be studied. Through the isolation of suppressor mutations, proteins which interact with the Mre proteins will be identified.