K/CL COTRANSPORTER

描述：研究人员最近克隆了一种蛋白质并对其进行了测序， 被称为KCC1，催化钾和氯化物的共同运输。 功能研究表明，红细胞中存在这种蛋白质。 和其他一些组织，但研究人员已经制造出了第一个 关于这个转运体的分子水平的信息。的具体目标 项目包括：1)确定蛋白质在离子结合中的重要区域 使用定点突变和嵌合体抑制环状利尿剂 K-Cl共转运体和Na-K-2Cl共转运体之间的关系；2) 通过确定磷酸化位点来表征KCC-1的调控， 检测与KCC1相互作用的蛋白质，并表征 细胞内氯的调节作用；3)KCC1在哺乳动物中的定位 组织，主要是吸收上皮；以及3)产生大量的 蓝藻相关的阳离子-氯离子共转运蛋白CCCcy 生产适合于结构分析的晶体的目的。

DESCRIPTION: The investigator has recently cloned and sequenced a protein, known as KCC1, that catalyzes the cotransport of potassium and chloride. Functional studies had indicated the presence of such a protein in red cells and a few other tissues, but the investigator has produced the first molecular-level information about this transporter. The specific aims of the project are 1) determine regions of the protein important in ion binding and loop-diuretic inhibition using site-directed mutagenesis and chimeras between the K-Cl cotransporter and the Na-K-2Cl cotransporter; 2) characterize regulation of KCC-1 by determining sites of phosphorylation, detecting proteins that interact with KCC1, and characterizing the regulatory role of intracellular chloride; 3) localize KCC1 in mammalian tissues, with focus on absorptive epithelia; and 3) produce large amounts of a related cyanobacterial cation-chloride cotransporter, CCCcy, for the purpose of producing crystals suitable for structural analysis.