BABOON CMV--DISEASE RISK AFTER XENOTRANSPLANTATION

狒狒 CMV——异种移植后的疾病风险

• 批准号: 2671441
• 负责人: Marian G Michaels
• 金额: $ 7.85万
• 依托单位: CHILDREN'S HOSP PITTSBURGH/UPMC HLTH SYS
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2671441
• 关键词: Herpesviridae disease; baboons; clinical research; communicable disease transmission; cytomegalovirus; disease /disorder proneness /risk; genetic strain; human subject; polymerase chain reaction; recombinant virus; restriction fragment length polymorphism; tissue /cell culture; transplantation immunology; virus infection mechanism; xenotransplantation

The proposed studies are designed to evaluate the potential for transmission of CMV between baboon and human following xenotransplantation. Specific Aim 1 will optimize CMV detection and identification methods, and will use these methods to analyze the spectrum of BCMV isolates which can be found in different animals. These studies will use newly developed RFLP and BCMV-specific PCR methods to selectively identify human CMV (HCMV) and BCMV and to identify strains of BCMV. Specific Aim 2 will seek in vitro evidence that HCMV and BCMV can recombine in co-transfection studies that use HCMV and BCMV with differential molecular markers. Specific Aim 3 will determine the immunologic factors that influence transmission of CMV across species barriers. These studies will focus on serologic and T cell reactivity to BCMV vs. HCMV. Specific Aim 4 will determine whether CMV can cross species barriers in vivo by infecting baboons with HCMV.

拟议的研究旨在评估 狒狒和人类之间的CMV传播 异种移植 具体目标1将优化CMV检测， 识别方法，并将使用这些方法来分析 可以在不同动物中发现的BCMV分离株的谱。 这些研究将使用新开发的RFLP和BCMV特异性PCR 选择性鉴定人CMV（HCMV）和BCMV的方法， 鉴定BCMV菌株。 具体目标2将寻求体外证据 HCMV和BCMV可以在共转染研究中重组， HCMV和BCMV具有差异分子标记。 第3章将 确定影响CMV传播的免疫学因素 跨越物种壁垒。 这些研究将集中在血清学和T 细胞对BCMV和HCMV的反应性。 具体目标4将决定 CMV是否可以通过感染狒狒来跨越体内的物种屏障， 巨细胞病毒。