BABOON CMV--DISEASE RISK AFTER XENOTRANSPLANTATION

拟议的研究旨在评估 巨细胞病毒在狒狒和人类跟随者之间的传播 异种移植。特定目标1将优化CMV检测和 识别方法，并将使用这些方法来分析 可在不同动物身上发现的BCMV分离物的谱系。 这些研究将使用新开发的RFLP和BCMV特异性PCR 方法选择性鉴定人巨细胞病毒(HCMV)和牛巨细胞病毒(BCMV) 鉴定BCMV的毒株。《特定目标2》将在体外寻找证据 巨细胞病毒和巨细胞病毒可以在共转染研究中重组 HCMV和BCMV的差异分子标记。具体目标3将 确定影响巨细胞病毒传播的免疫学因素 跨越物种障碍。这些研究将集中在血清学和T细胞 细胞对BCMV和HCMV的反应性。具体目标4将决定 巨细胞病毒是否可以通过感染狒狒体内跨越物种障碍 人巨细胞病毒。

The proposed studies are designed to evaluate the potential for transmission of CMV between baboon and human following xenotransplantation. Specific Aim 1 will optimize CMV detection and identification methods, and will use these methods to analyze the spectrum of BCMV isolates which can be found in different animals. These studies will use newly developed RFLP and BCMV-specific PCR methods to selectively identify human CMV (HCMV) and BCMV and to identify strains of BCMV. Specific Aim 2 will seek in vitro evidence that HCMV and BCMV can recombine in co-transfection studies that use HCMV and BCMV with differential molecular markers. Specific Aim 3 will determine the immunologic factors that influence transmission of CMV across species barriers. These studies will focus on serologic and T cell reactivity to BCMV vs. HCMV. Specific Aim 4 will determine whether CMV can cross species barriers in vivo by infecting baboons with HCMV.