STUDIES OF CHLAMYDIA TRACHOMATIS
STUDIES OF CHLAMYDIA TRACHOMATIS
批准号:
6099529
负责人:
Priscilla B. Wyrick
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-07-01 至 1999-06-30
关键词:
Chlamydia trachomatis antigen presentation apical membrane bacterial antigens bacterial proteins basolateral membrane brefeldin A cellular polarity cooperative study endometrium epithelium fluorescence microscopy glycolipids histocompatibility antigens host organism interaction human tissue immunoelectron microscopy inclusion body lipopolysaccharides membrane proteins microorganism immunology secretion stress proteins surface antigens tissue /cell culture
中文摘要
沙眼衣原体是细菌性感染的主要原因
英文摘要
C. trachomatis is the leading cause of bacerially-acquired sexually
transmitted infections in the USA and is responsible for 25-50% of the
estimate 1 million cases per year of pelvic inflammatory disease.
Sequelae include ectopic pregnancy and sterility. To begin to dissect the
destructive versus the protective response as well as identify potential
vaccine candidates, our overall goal is to understand the cell and
molecular biology of chlamydial antigen sorting and secretion in infected
polarized human endometrial epithelial cells.
We have detected escape of chlamydial LPS and the major outer membrane
protein (MOMP) to the surface of infected cells prior to the natural
release of chlamydiae at the end of the developmental cycle. Do
chlamydial stress response proteins also escape to the infected cell
surface: What pathway do these antigens take from the inclusion and how
are the surface exposed antigens perceived by the host immune response:
We shall use the powerful technique of post-embedding immunoelectron
microscopy on Lowicryl-processed samples to determine in Specific Aim 1 if
C. trachomatis serovar E GroEL and DnaK are also secreted to the surface
of normally infected and penicillin and interferon gamma-induced
persistently infected human endometrial epithelial cells, and in Specific
Aim 2 to continue to assess whether or not chlamydial antigens are
secreted in a polarized direction. Primary antibodies (ABY) directed
against chlamydia specific histones will serve as a control marker for
antigens which do not escape the inclusion and mABY directed against
Class 1 molecules will serve as a marker for the basolateral domain.
Digitized fluorescence microscopy of duplicate samples will give a more
quantitative assessment of the pattern of directed antigen secretion. In
Specific Aim 3, we shall investigate if chlamydial surface antigen
secretion is modulated by estrogen and progesterone. Since the trans-
golgi excytosis inhibitor Brefeldin A reduces escape of LPS and MOMP from
inclusions, chlamydial antigens may exploit the secretory pathway to reach
the infected cell surface. In Specific Aim 4, we shall analyze the
pathway of chlamydial antigen sorting by co-localizing escaped chlamydial
antigen with the golgi markers beta-COP, p58 and C6-NBD-ceramide.
Finally, to begin to examine immune control of chlamydial growth, we have
established an in vitro co-cultivation model to assess host effector PMN
and T cell responses to infected human epithelial cells.
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会议论文
EFFICACY OF MICROBICIDES IN CHLAMYDIA TRACHOMATIS
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批准号:6352614
-
项目类别:
-
资助金额:$5.82万
-
财政年份:2000
-
负责人:Priscilla B. Wyrick
-
依托单位:
EVALUATION OF C31G VS NONOXYNOL-9 AS TOPICAL MICROBICIDES
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批准号:6099894
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项目类别:
-
资助金额:$10.86万
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财政年份:1998
-
负责人:Priscilla B. Wyrick
-
依托单位:
EVALUATION OF C31G VS NONOXYNOL-9 AS TOPICAL MICROBICIDES
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批准号:6235313
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项目类别:
-
资助金额:$11.86万
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财政年份:1997
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负责人:Priscilla B. Wyrick
-
依托单位:
STUDIES OF CHLAMYDIA TRACHOMATIS
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批准号:6235018
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项目类别:
-
资助金额:$18.22万
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财政年份:1997
-
负责人:Priscilla B. Wyrick
-
依托单位:
EFFICACY OF MICROBICIDES IN CHLAMYDIA TRACHOMATIS
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批准号:6233362
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项目类别:
-
资助金额:$5.82万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:6372959
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项目类别:
-
资助金额:$30.19万
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财政年份:1995
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负责人:Priscilla B. Wyrick
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依托单位:
Virulence Factors of Chlamydiae
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批准号:6913865
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项目类别:
-
资助金额:$33.15万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:2059974
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项目类别:
-
资助金额:$25.2万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
Virulence Factors of Chlamydiae
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批准号:7588794
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项目类别:
-
资助金额:$42.95万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:2059976
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项目类别:
-
资助金额:$25.51万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
Virulence Factors of Chlamydiae
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批准号:7081423
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项目类别:
-
资助金额:$41.26万
-
财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:6709332
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项目类别:
-
资助金额:$32.63万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:6631633
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项目类别:
-
资助金额:$31.78万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
Virulence Factors of Chlamydiae
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批准号:7196436
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项目类别:
-
资助金额:$41.27万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:2886350
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项目类别:
-
资助金额:$27.91万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:2390231
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项目类别:
-
资助金额:$26.21万
-
财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:6094549
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项目类别:
-
资助金额:$29.78万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:2671678
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项目类别:
-
资助金额:$27.15万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
-
依托单位:
VIRULENCE FACTORS OF CHLAMYDIAE
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批准号:6510084
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项目类别:
-
资助金额:$30.95万
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财政年份:1995
-
负责人:Priscilla B. Wyrick
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依托单位:
Virulence Factors of Chlamydiae
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批准号:7387359
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项目类别:
-
资助金额:$41.7万
-
财政年份:1995
-
负责人:Priscilla B. Wyrick
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依托单位:
海外基金