METABOLIC CONTROL OF FEEDING BEHAVIOR

进食行为的代谢控制

• 批准号: 2639736
• 负责人: MARK FRIEDMAN
• 金额: $ 28.99万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2639736
• 关键词: adenosine triphosphate; antimetabolites; behavior prediction; bioenergetics; calcium flux; carbohydrate analog; laboratory rat; liver cells; liver metabolism; mannitol; nuclear magnetic resonance spectroscopy; nutrient intake activity; nutrition related tag; ouabain; psychobiology; sodium potassium exchanging ATPase

This is a request for five additional years support to carry out experiments involving metabolic subcellular changes in the liver and how they influence the onset of meals in rats. Prior evidence produced by the investigator suggests that when food intake is initiated after the administration of the metabolic inhibitor, 2, 5-AM, there are several correlates in hepatocytes that may well have a causal role. More specifically, the results of several types of experiments have converged to strongly implicate that the liver is the target for 2, 5-AMs or exigenicaction; and other experiments have indicated that a key factor (or at least close correlate) is a reduction in the amount of ATP in hepatocytes. Proposed experiments will take the analysis to a more reductionistic level by assessing whether bound or free cellular ATP is more correlated with eating and by then determining if a reduction in the key ATP pool elicit seating when induced by other means; will determine if other drugs thought to elicit eating via the liver (e.g., methyl palmoxirate) are associated with similar hepatocyte changes; will assess the hypothesis that the signal to eat is associated with functioning of the hepatocyte sodium pump; and will determine if changes in hepatocyte calcium pools are a means for generating a signal that could pass from hepatocytes to other cells and on the CNS.

这是一项额外五年支持的请求 涉及肝脏代谢亚细胞变化的实验以及如何 它们影响大鼠的进餐时间。先前提供的证据 研究人员建议，当进食后开始 施用代谢抑制剂 2, 5-AM，有几种 与肝细胞相关，很可能具有因果作用。更多的 具体来说，几种类型的实验的结果已经收敛 强烈暗示肝脏是 2、5-AM 或 外生作用；和其他实验表明，一个关键因素（或 至少密切相关）是 ATP 量的减少 肝细胞。拟议的实验将使分析更加深入 通过评估结合或游离细胞 ATP 是否还原水平 与饮食更相关，然后确定是否减少 关键 ATP 池在通过其他方式诱导时会引发座位；将确定是否 其他被认为通过肝脏进食的药物（例如甲基 palmoxirate）与类似的肝细胞变化相关；将评估 假设进食信号与大脑功能有关 肝细胞钠泵；并确定肝细胞是否发生变化 钙池是一种产生信号的方法，该信号可以从 肝细胞到其他细胞和中枢神经系统。